Functional characterisation of new sesquiterpene synthase from the Malaysian herbal plant, polygonum minus

Nor Azizun Rusdi, Hoe Han Goh, Suriana Sabri, Ramzi Ahmad Bazli, Normah Mohd. Noor, Syarul Nataqain Baharum

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Polygonum minus (syn. Persicaria minor) is a herbal plant that is well known for producing sesquiterpenes, which contribute to its flavour and fragrance. This study describes the cloning and functional characterisation of PmSTPS1 and PmSTPS2, two sesquiterpene synthase genes that were identified from P. minus transcriptome data mining. The full-length sequences of the PmSTPS1 and PmSTPS2 genes were expressed in the E. coli pQE-2 expression vector. The sizes of PmSTPS1 and PmSTPS2 were 1098 bp and 1967 bp, respectively, with open reading frames (ORF) of 1047 and 1695 bp and encoding polypeptides of 348 and 564 amino acids, respectively. The proteins consist of three conserved motifs, namely, Asp-rich substrate binding (DDxxD), metal binding residues (NSE/DTE), and cytoplasmic ER retention (RxR), as well as the terpene synthase family N-terminal domain and C-terminal metal-binding domain. From the in vitro enzyme assays, using the farnesyl pyrophosphate (FPP) substrate, the PmSTPS1 enzyme produced multiple acyclic sesquiterpenes of β-farnesene, α-farnesene, and farnesol, while the PmSTPS2 enzyme produced an additional nerolidol as a final product. The results confirmed the roles of PmSTPS1 and PmSTPS2 in the biosynthesis pathway of P. minus, to produce aromatic sesquiterpenes.

Original languageEnglish
Article number1370
JournalMolecules
Volume23
Issue number6
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Polygonum
Sesquiterpenes
enzymes
genes
terpenes
data mining
biosynthesis
Enzymes
Genes
Metals
polypeptides
Farnesol
Fragrances
metals
Data Mining
amino acids
Cloning
Flavors
Biosynthesis
Enzyme Assays

Keywords

  • Farnesol
  • Nerolidol
  • Polygonum minus
  • Sesquiterpene synthase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Functional characterisation of new sesquiterpene synthase from the Malaysian herbal plant, polygonum minus. / Rusdi, Nor Azizun; Goh, Hoe Han; Sabri, Suriana; Ahmad Bazli, Ramzi; Mohd. Noor, Normah; Baharum, Syarul Nataqain.

In: Molecules, Vol. 23, No. 6, 1370, 01.01.2018.

Research output: Contribution to journalArticle

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