Functional analysis of the Elaeis oleifera scsquitcrpcnc synthase promoter reveals non-specific tissue expression and activation under stress condition

Ismanizan Ismail, Norazreen Abdul Rahman, Chan Kok Fei, Zamri Zainal, Nik Marzuki Sidik, Che Radziah Che Mohd. Zain

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3 Citations (Scopus)

Abstract

This research aimed to evaluate the specificity of sesquiterpene synthase promoter (SesqPro) activity in the oil palm tissues and tomato hairy roots and to determine the functional region of the promoter The effect of jasmonic acid (JA) on the promoter activation and gene expression was also analyzed A series of 5' sequence deletions on the full-length SesqPro were generated and individually cloned into the pCAMBIA 1301 vector. Functional analysis was carried out on leaves, mesocarp slices and Immature Embryos (IE) of oil palm and tomato hairy roots that had been transformed with full-length SesqPro (PSPr-VF6). GUS expression was found in all the tissues and a higher activity was detected in IE and mesocarp slices. All the constructed derivatives of SesqPro were transformed into IE and mesocarp slices in order to determine the promoter regions which are responsible for £cne expression. The reduction of GUS activity was found to be related to the removal of DNA sequences within the promoter region. The promoter was induced by the elicitor molecule JA, thus suggesting the presence of JA responsive elements within the promoter. Incubation with 100 μM of JA showed higher GUS activity in IE and mesocarp slices that had been transformed with PSPr-VF4 to PSPr-VF6. Nevertheless, the GUS activity was drastically reduced in IE and mesocarp slices containing the PSPr-VF3 promoter, suggesting that the presence of the G/A hybrid bost located at -622 to -617 act as a specific element in Ksponse to elicitors. This study has shown that the action of SesqPro is non-specific and was influenced by JA induction.

Original languageEnglish
Pages (from-to)24-37
Number of pages14
JournalAmerican Journal of Plant Physiology
Volume4
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Elaeis oleifera
Sesquiterpenes
Embryonic Structures
promoter regions
Genetic Promoter Regions
mesocarp
jasmonic acid
Lycopersicon esculentum
embryo (plant)
sesquiterpenoids
Oils
immatures
Sequence Deletion
Solanum habrochaites
Elaeis guineensis
tissues
Gene Expression
Research

Keywords

  • Elicitor induction
  • GUS expression
  • Hairy roots
  • Jasmonic acid
  • Phytoalexin
  • Promoter deletion

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

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title = "Functional analysis of the Elaeis oleifera scsquitcrpcnc synthase promoter reveals non-specific tissue expression and activation under stress condition",
abstract = "This research aimed to evaluate the specificity of sesquiterpene synthase promoter (SesqPro) activity in the oil palm tissues and tomato hairy roots and to determine the functional region of the promoter The effect of jasmonic acid (JA) on the promoter activation and gene expression was also analyzed A series of 5' sequence deletions on the full-length SesqPro were generated and individually cloned into the pCAMBIA 1301 vector. Functional analysis was carried out on leaves, mesocarp slices and Immature Embryos (IE) of oil palm and tomato hairy roots that had been transformed with full-length SesqPro (PSPr-VF6). GUS expression was found in all the tissues and a higher activity was detected in IE and mesocarp slices. All the constructed derivatives of SesqPro were transformed into IE and mesocarp slices in order to determine the promoter regions which are responsible for £cne expression. The reduction of GUS activity was found to be related to the removal of DNA sequences within the promoter region. The promoter was induced by the elicitor molecule JA, thus suggesting the presence of JA responsive elements within the promoter. Incubation with 100 μM of JA showed higher GUS activity in IE and mesocarp slices that had been transformed with PSPr-VF4 to PSPr-VF6. Nevertheless, the GUS activity was drastically reduced in IE and mesocarp slices containing the PSPr-VF3 promoter, suggesting that the presence of the G/A hybrid bost located at -622 to -617 act as a specific element in Ksponse to elicitors. This study has shown that the action of SesqPro is non-specific and was influenced by JA induction.",
keywords = "Elicitor induction, GUS expression, Hairy roots, Jasmonic acid, Phytoalexin, Promoter deletion",
author = "Ismanizan Ismail and Rahman, {Norazreen Abdul} and Fei, {Chan Kok} and Zamri Zainal and Sidik, {Nik Marzuki} and {Che Mohd. Zain}, {Che Radziah}",
year = "2009",
doi = "10.3923/ajpp.2009.24.37",
language = "English",
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pages = "24--37",
journal = "American Journal of Plant Physiology",
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T1 - Functional analysis of the Elaeis oleifera scsquitcrpcnc synthase promoter reveals non-specific tissue expression and activation under stress condition

AU - Ismail, Ismanizan

AU - Rahman, Norazreen Abdul

AU - Fei, Chan Kok

AU - Zainal, Zamri

AU - Sidik, Nik Marzuki

AU - Che Mohd. Zain, Che Radziah

PY - 2009

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N2 - This research aimed to evaluate the specificity of sesquiterpene synthase promoter (SesqPro) activity in the oil palm tissues and tomato hairy roots and to determine the functional region of the promoter The effect of jasmonic acid (JA) on the promoter activation and gene expression was also analyzed A series of 5' sequence deletions on the full-length SesqPro were generated and individually cloned into the pCAMBIA 1301 vector. Functional analysis was carried out on leaves, mesocarp slices and Immature Embryos (IE) of oil palm and tomato hairy roots that had been transformed with full-length SesqPro (PSPr-VF6). GUS expression was found in all the tissues and a higher activity was detected in IE and mesocarp slices. All the constructed derivatives of SesqPro were transformed into IE and mesocarp slices in order to determine the promoter regions which are responsible for £cne expression. The reduction of GUS activity was found to be related to the removal of DNA sequences within the promoter region. The promoter was induced by the elicitor molecule JA, thus suggesting the presence of JA responsive elements within the promoter. Incubation with 100 μM of JA showed higher GUS activity in IE and mesocarp slices that had been transformed with PSPr-VF4 to PSPr-VF6. Nevertheless, the GUS activity was drastically reduced in IE and mesocarp slices containing the PSPr-VF3 promoter, suggesting that the presence of the G/A hybrid bost located at -622 to -617 act as a specific element in Ksponse to elicitors. This study has shown that the action of SesqPro is non-specific and was influenced by JA induction.

AB - This research aimed to evaluate the specificity of sesquiterpene synthase promoter (SesqPro) activity in the oil palm tissues and tomato hairy roots and to determine the functional region of the promoter The effect of jasmonic acid (JA) on the promoter activation and gene expression was also analyzed A series of 5' sequence deletions on the full-length SesqPro were generated and individually cloned into the pCAMBIA 1301 vector. Functional analysis was carried out on leaves, mesocarp slices and Immature Embryos (IE) of oil palm and tomato hairy roots that had been transformed with full-length SesqPro (PSPr-VF6). GUS expression was found in all the tissues and a higher activity was detected in IE and mesocarp slices. All the constructed derivatives of SesqPro were transformed into IE and mesocarp slices in order to determine the promoter regions which are responsible for £cne expression. The reduction of GUS activity was found to be related to the removal of DNA sequences within the promoter region. The promoter was induced by the elicitor molecule JA, thus suggesting the presence of JA responsive elements within the promoter. Incubation with 100 μM of JA showed higher GUS activity in IE and mesocarp slices that had been transformed with PSPr-VF4 to PSPr-VF6. Nevertheless, the GUS activity was drastically reduced in IE and mesocarp slices containing the PSPr-VF3 promoter, suggesting that the presence of the G/A hybrid bost located at -622 to -617 act as a specific element in Ksponse to elicitors. This study has shown that the action of SesqPro is non-specific and was influenced by JA induction.

KW - Elicitor induction

KW - GUS expression

KW - Hairy roots

KW - Jasmonic acid

KW - Phytoalexin

KW - Promoter deletion

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