Fresh soy oil protects against vascular changes in an estrogen-deficient rat model

An electron microscopy study

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: To observe the effects of consuming repeatedly heated soy oil on the aortic tissues of estrogen-deficient rats. Methods: Thirty female Sprague Dawley rats (200- 250 g) were divided equally into five groups. One group served as the normal control (NC) group. The four treated groups were ovariectomized and were fed as follows: 2% cholesterol diet (OVXC); 2% cholesterol diet + fresh soy oil (FSO); 2% cholesterol diet + once-heated soy oil (1HSO); and 2% cholesterol diet + five-times-heated soy oil (5HSO). After four months, the rats were sacrificed, and the aortic tissues were obtained for histological studies. Results: After four months of feeding, the NC, FSO and 1HSO groups had a lower body weight gain compared to the OVXC and 5HSO groups. The tunica intima/media ratio in the 5HSO group was significantly thicker (p < 0.05) compared to the NC, OVXC and FSO groups. Electron microscopy showed that endothelial cells were normally shaped in the FSO and NC groups but irregular in the 1HSO and 5HSO groups. A greater number of collagen fibers and vacuoles were observed in the 5HSO group compared to the other treatment groups. Conclusions: Fresh soy oil offered protection in the estrogen-deficient state, as these rats had similar features to those of the NC group. The damage to the tunica intima and the increase in the ratio of tunica intima/media thickness showed the deleterious effect of consuming repeatedly heated soy oil in castrated female rats.

Original languageEnglish
Pages (from-to)1113-1119
Number of pages7
JournalClinics
Volume64
Issue number11
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Blood Vessels
Electron Microscopy
Estrogens
Oils
Tunica Intima
Cholesterol
Diet
Tunica Media
Control Groups
Vacuoles
Weight Gain
Sprague Dawley Rats
Collagen
Endothelial Cells
Body Weight

Keywords

  • Aorta
  • Atherosclerosis
  • Estrogen deficiency
  • Heating
  • Soy oil

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fresh soy oil protects against vascular changes in an estrogen-deficient rat model : An electron microscopy study. / Adam, Siti Khadijah; Das, Srijit; Othman, Faizah; Jaarin, Kamsiah.

In: Clinics, Vol. 64, No. 11, 11.2009, p. 1113-1119.

Research output: Contribution to journalArticle

@article{281d396a17bb4094a83d2080a1dc0d3a,
title = "Fresh soy oil protects against vascular changes in an estrogen-deficient rat model: An electron microscopy study",
abstract = "Objective: To observe the effects of consuming repeatedly heated soy oil on the aortic tissues of estrogen-deficient rats. Methods: Thirty female Sprague Dawley rats (200- 250 g) were divided equally into five groups. One group served as the normal control (NC) group. The four treated groups were ovariectomized and were fed as follows: 2{\%} cholesterol diet (OVXC); 2{\%} cholesterol diet + fresh soy oil (FSO); 2{\%} cholesterol diet + once-heated soy oil (1HSO); and 2{\%} cholesterol diet + five-times-heated soy oil (5HSO). After four months, the rats were sacrificed, and the aortic tissues were obtained for histological studies. Results: After four months of feeding, the NC, FSO and 1HSO groups had a lower body weight gain compared to the OVXC and 5HSO groups. The tunica intima/media ratio in the 5HSO group was significantly thicker (p < 0.05) compared to the NC, OVXC and FSO groups. Electron microscopy showed that endothelial cells were normally shaped in the FSO and NC groups but irregular in the 1HSO and 5HSO groups. A greater number of collagen fibers and vacuoles were observed in the 5HSO group compared to the other treatment groups. Conclusions: Fresh soy oil offered protection in the estrogen-deficient state, as these rats had similar features to those of the NC group. The damage to the tunica intima and the increase in the ratio of tunica intima/media thickness showed the deleterious effect of consuming repeatedly heated soy oil in castrated female rats.",
keywords = "Aorta, Atherosclerosis, Estrogen deficiency, Heating, Soy oil",
author = "Adam, {Siti Khadijah} and Srijit Das and Faizah Othman and Kamsiah Jaarin",
year = "2009",
month = "11",
doi = "10.1590/S1807-59322009001100012",
language = "English",
volume = "64",
pages = "1113--1119",
journal = "Clinics",
issn = "1807-5932",
publisher = "University of Sao Paolo",
number = "11",

}

TY - JOUR

T1 - Fresh soy oil protects against vascular changes in an estrogen-deficient rat model

T2 - An electron microscopy study

AU - Adam, Siti Khadijah

AU - Das, Srijit

AU - Othman, Faizah

AU - Jaarin, Kamsiah

PY - 2009/11

Y1 - 2009/11

N2 - Objective: To observe the effects of consuming repeatedly heated soy oil on the aortic tissues of estrogen-deficient rats. Methods: Thirty female Sprague Dawley rats (200- 250 g) were divided equally into five groups. One group served as the normal control (NC) group. The four treated groups were ovariectomized and were fed as follows: 2% cholesterol diet (OVXC); 2% cholesterol diet + fresh soy oil (FSO); 2% cholesterol diet + once-heated soy oil (1HSO); and 2% cholesterol diet + five-times-heated soy oil (5HSO). After four months, the rats were sacrificed, and the aortic tissues were obtained for histological studies. Results: After four months of feeding, the NC, FSO and 1HSO groups had a lower body weight gain compared to the OVXC and 5HSO groups. The tunica intima/media ratio in the 5HSO group was significantly thicker (p < 0.05) compared to the NC, OVXC and FSO groups. Electron microscopy showed that endothelial cells were normally shaped in the FSO and NC groups but irregular in the 1HSO and 5HSO groups. A greater number of collagen fibers and vacuoles were observed in the 5HSO group compared to the other treatment groups. Conclusions: Fresh soy oil offered protection in the estrogen-deficient state, as these rats had similar features to those of the NC group. The damage to the tunica intima and the increase in the ratio of tunica intima/media thickness showed the deleterious effect of consuming repeatedly heated soy oil in castrated female rats.

AB - Objective: To observe the effects of consuming repeatedly heated soy oil on the aortic tissues of estrogen-deficient rats. Methods: Thirty female Sprague Dawley rats (200- 250 g) were divided equally into five groups. One group served as the normal control (NC) group. The four treated groups were ovariectomized and were fed as follows: 2% cholesterol diet (OVXC); 2% cholesterol diet + fresh soy oil (FSO); 2% cholesterol diet + once-heated soy oil (1HSO); and 2% cholesterol diet + five-times-heated soy oil (5HSO). After four months, the rats were sacrificed, and the aortic tissues were obtained for histological studies. Results: After four months of feeding, the NC, FSO and 1HSO groups had a lower body weight gain compared to the OVXC and 5HSO groups. The tunica intima/media ratio in the 5HSO group was significantly thicker (p < 0.05) compared to the NC, OVXC and FSO groups. Electron microscopy showed that endothelial cells were normally shaped in the FSO and NC groups but irregular in the 1HSO and 5HSO groups. A greater number of collagen fibers and vacuoles were observed in the 5HSO group compared to the other treatment groups. Conclusions: Fresh soy oil offered protection in the estrogen-deficient state, as these rats had similar features to those of the NC group. The damage to the tunica intima and the increase in the ratio of tunica intima/media thickness showed the deleterious effect of consuming repeatedly heated soy oil in castrated female rats.

KW - Aorta

KW - Atherosclerosis

KW - Estrogen deficiency

KW - Heating

KW - Soy oil

UR - http://www.scopus.com/inward/record.url?scp=71449120703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71449120703&partnerID=8YFLogxK

U2 - 10.1590/S1807-59322009001100012

DO - 10.1590/S1807-59322009001100012

M3 - Article

VL - 64

SP - 1113

EP - 1119

JO - Clinics

JF - Clinics

SN - 1807-5932

IS - 11

ER -