Fourth-Generation Antibiotic Gatifloxacin Encapsulated by Microemulsions

Structural and Probing Dynamics

Muhammad Faizan Nazar, Muhammad Yasir Siddique, Muhammad Atif Saleem, Muddassar Zafar, Faisal Nawaz, Muhammad Ashfaq, Asad Muhammad Khan, Hafiz Muhammad Abd Ur Rahman, Muhammad Bilal Tahir, Mohamad Azwani Shah Mat Lazim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To overcome the increased disease rate, utilization of the versatile broad spectrum antibiotic drugs in controlled drug-delivery systems has been a challenging and complex consignment. However, with the development of microemulsion (μE)-based formulations, drugs can be effectively encapsulated and transferred to the target source. Herein, two biocompatible oil-in-water (o/w) μE formulations comprising clove oil/Tween 20/ethylene glycol/water (formulation A) and clove oil/Tween 20/1-butanol/water (formulation B) were developed for encapsulating the gatifloxacin (GTF), a fourth-generation antibiotic. The pseudoternary phase diagrams were mapped at a constant surfactant/co-surfactant (1:1) ratio to bound the existence of a monophasic isotropic region for as-formulated μEs. Multiple complementary characterization techniques, namely, conductivity (σ), viscosity (η), and optical microscopy analyses, were used to study the gradual changes that occurred in the microstructure of the as-formulated μEs, indicating the presence of a percolation transformation to a bicontinuous permeate flow. GTF showed good solubility, 3.2 wt % at pH 6.2 and 4.0 wt % at pH 6.8, in optimum μE of formulation A and formulation B, respectively. Each loaded μE formulation showed long-term stability over 8 months of storage. Moreover, no observable aggregation of GTF was found, as revealed by scanning transmission electron microscopy and peak-to-peak correlation of IR analysis, indicating the stability of GTF inside the formulation. The average particle size of each μE, measured by dynamic light scattering, increased upon loading GTF, intending the accretion of drug in the interfacial layers of microdomains. Likewise, fluorescence probing sense an interfacial hydrophobic environment to GTF molecules in any of the examined formulations, which may be of significant interest for understanding the kinetics of drug release.

Original languageEnglish
Pages (from-to)10603-10612
Number of pages10
JournalLangmuir
Volume34
Issue number36
DOIs
Publication statusPublished - 11 Sep 2018

Fingerprint

dynamic structural analysis
antibiotics
Microemulsions
Antibiotics
Anti-Bacterial Agents
formulations
Surface active agents
Clove Oil
Controlled drug delivery
drugs
Water
Polysorbates
Dynamic light scattering
Ethylene glycol
Butenes
Surface-Active Agents
Phase diagrams
Optical microscopy
Agglomeration
Solubility

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Fourth-Generation Antibiotic Gatifloxacin Encapsulated by Microemulsions : Structural and Probing Dynamics. / Nazar, Muhammad Faizan; Yasir Siddique, Muhammad; Saleem, Muhammad Atif; Zafar, Muddassar; Nawaz, Faisal; Ashfaq, Muhammad; Khan, Asad Muhammad; Abd Ur Rahman, Hafiz Muhammad; Tahir, Muhammad Bilal; Mat Lazim, Mohamad Azwani Shah.

In: Langmuir, Vol. 34, No. 36, 11.09.2018, p. 10603-10612.

Research output: Contribution to journalArticle

Nazar, MF, Yasir Siddique, M, Saleem, MA, Zafar, M, Nawaz, F, Ashfaq, M, Khan, AM, Abd Ur Rahman, HM, Tahir, MB & Mat Lazim, MAS 2018, 'Fourth-Generation Antibiotic Gatifloxacin Encapsulated by Microemulsions: Structural and Probing Dynamics', Langmuir, vol. 34, no. 36, pp. 10603-10612. https://doi.org/10.1021/acs.langmuir.8b01775
Nazar MF, Yasir Siddique M, Saleem MA, Zafar M, Nawaz F, Ashfaq M et al. Fourth-Generation Antibiotic Gatifloxacin Encapsulated by Microemulsions: Structural and Probing Dynamics. Langmuir. 2018 Sep 11;34(36):10603-10612. https://doi.org/10.1021/acs.langmuir.8b01775
Nazar, Muhammad Faizan ; Yasir Siddique, Muhammad ; Saleem, Muhammad Atif ; Zafar, Muddassar ; Nawaz, Faisal ; Ashfaq, Muhammad ; Khan, Asad Muhammad ; Abd Ur Rahman, Hafiz Muhammad ; Tahir, Muhammad Bilal ; Mat Lazim, Mohamad Azwani Shah. / Fourth-Generation Antibiotic Gatifloxacin Encapsulated by Microemulsions : Structural and Probing Dynamics. In: Langmuir. 2018 ; Vol. 34, No. 36. pp. 10603-10612.
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