Formulation of defined media for carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth characteristics of the bacterium

In Seop Chang, Byung Hong Kim, Do Hee Kim, Robert W. Lovitt, Ha Chin Sung

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Phosphate-buffered (PBBM) and carbonate-buffered (CBBM) basal media were used in the formulation of defined media for the cultivation of Eubacterium limosum KIST612 with carbon monoxide (CO) as the sole energy source. The bacterium was adapted to the minimal media by sequential passage in media containing casamino acids and those containing ammonium chloride in the place of yeast extract. Biological growth was slower with a lower growth yield in the defined minimal media than in PBBM or CBBM. More butyrate was produced in phosphate-buffered media than in carbonate-buffered media. The bacteria grew without any organic nitrogen in the presence of trace quantities of biotin and pantothenic acid. Anaerobic digester fluid stimulated bacterial growth.

Original languageEnglish
Pages (from-to)682-685
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume88
Issue number6
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Eubacterium
Carbon Monoxide
Carbon monoxide
Fermentation
Bacteria
Carbonates
Phosphates
Growth
Pantothenic Acid
Ammonium Chloride
Acids
Butyrates
Biotin
Yeast
Nitrogen
Yeasts
Fluids

Keywords

  • Acetogen
  • CO fermentation
  • Defined medium
  • Eubacterium limosum

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Formulation of defined media for carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth characteristics of the bacterium. / Chang, In Seop; Kim, Byung Hong; Kim, Do Hee; Lovitt, Robert W.; Sung, Ha Chin.

In: Journal of Bioscience and Bioengineering, Vol. 88, No. 6, 1999, p. 682-685.

Research output: Contribution to journalArticle

Chang, In Seop ; Kim, Byung Hong ; Kim, Do Hee ; Lovitt, Robert W. ; Sung, Ha Chin. / Formulation of defined media for carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth characteristics of the bacterium. In: Journal of Bioscience and Bioengineering. 1999 ; Vol. 88, No. 6. pp. 682-685.
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