Formulation of abalone hemocyanin with high antiviral activity and stability

Negar Talaei Zanjani, Mohd Fareed Mohd Sairi, Gavin Marshall, Monica Miranda Saksena, Peter Valtchev, Vincent G. Gomes, Anthony L. Cunningham, Fariba Dehghani

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hemocyanin has been shown to have potential antiviral activity against herpes simplex virus type-1. However, current liquid formulations have short shelf life and high risk of bacterial contamination. The aim of our study was to develop a stable functional formulation. Analytical techniques (nano-differential scanning calorimetry and spectroscopy) and biological assays (cytotoxicity and plaque reduction) were employed to measure the effect of sugar addition on the physical properties and shelf life of the solid formulated hemocyanin. Sucrose improved thermal stability significantly by both increasing the aggregation onset temperature (70 C to >78 C) and enhancing the activation energy (18%). Lyophilisation without trehalose caused degradation and unfolding of the α-helices of hemocyanin. However, the addition of an optimal proportion of trehalose:protein (5:1 by weight) prevented the degradation and unfolding during lyophilisation, hence maintained the protein solubility. The estimated ED50 values of the formulated solid (0.43 ± 0.1) and liquid samples (0.37 ± 0.06) were similar in magnitude, and were significantly lower than the respective controls; thus, confirming enhanced antiviral activity of the formulation. Formulated compounds were stable for six months at 5 C storage. The enhanced shelf life and stable antiviral activity of the formulation offers its significant potential as effective therapeutic agent in future clinical applications.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalEuropean Journal of Pharmaceutical Sciences
Volume53
Issue number1
DOIs
Publication statusPublished - 12 Mar 2014
Externally publishedYes

Fingerprint

Hemocyanin
Antiviral Agents
Trehalose
Freeze Drying
Differential Scanning Calorimetry
Human Herpesvirus 1
Biological Assay
Solubility
Sucrose
Spectrum Analysis
Proteins
Hot Temperature
Weights and Measures
Temperature
Therapeutics

Keywords

  • Glycoprotein
  • HSV-1
  • Secondary structure
  • Thermal stability

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Formulation of abalone hemocyanin with high antiviral activity and stability. / Zanjani, Negar Talaei; Mohd Sairi, Mohd Fareed; Marshall, Gavin; Saksena, Monica Miranda; Valtchev, Peter; Gomes, Vincent G.; Cunningham, Anthony L.; Dehghani, Fariba.

In: European Journal of Pharmaceutical Sciences, Vol. 53, No. 1, 12.03.2014, p. 77-85.

Research output: Contribution to journalArticle

Zanjani, NT, Mohd Sairi, MF, Marshall, G, Saksena, MM, Valtchev, P, Gomes, VG, Cunningham, AL & Dehghani, F 2014, 'Formulation of abalone hemocyanin with high antiviral activity and stability', European Journal of Pharmaceutical Sciences, vol. 53, no. 1, pp. 77-85. https://doi.org/10.1016/j.ejps.2013.11.013
Zanjani, Negar Talaei ; Mohd Sairi, Mohd Fareed ; Marshall, Gavin ; Saksena, Monica Miranda ; Valtchev, Peter ; Gomes, Vincent G. ; Cunningham, Anthony L. ; Dehghani, Fariba. / Formulation of abalone hemocyanin with high antiviral activity and stability. In: European Journal of Pharmaceutical Sciences. 2014 ; Vol. 53, No. 1. pp. 77-85.
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