Formulation and optimization of levofloxacin loaded chitosan nanoparticle for ocular delivery: In-vitro characterization, ocular tolerance and antibacterial activity

Ameeduzzafar, Syed Sarim Imam, Bukhari Syed Nasir Abbas, Javed Ahmad, Asgar Ali

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, chitosan nanoparticles (CH-NPs) were used to encapsulate antibacterial agent levofloxacin for the treatment of ocular infection. The chitosan nanoparticles were prepared by ionic gelation method using chitosan and sodium tripolyphosphate. The developed formulations were evaluated for various physicochemical parameters and optimized formulation was converted into the sol-gel system to enhance the corneal residence time. The developed formulations showed the particle size in nanometric range with high encapsulation, and loading. HET-CAM test and histopathology of cornea demonstrated that optimized formulation was found to be non-irritant and safe for topical ophthalmic use. The antimicrobial study revealed that the developed formulation possesses higher antibacterial activity against P. aeruginosa, and S. aureus. The pharmacoscintigraphic study result revealed the reduced corneal clearance, naso-lachrymal drainage as well as higher retention of LFX in comparison to LFX solution. Our results concluded that levofloxacin loaded chitosan nanoparticles in situ gel system found to be an efficient carrier for ocular delivery of levofloxacin.

Original languageEnglish
Pages (from-to)650-659
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume108
DOIs
Publication statusPublished - 1 Mar 2018
Externally publishedYes

Fingerprint

Levofloxacin
Chitosan
Nanoparticles
Gels
Eye Infections
Polymethyl Methacrylate
Computer aided manufacturing
Gelation
Encapsulation
Particle Size
Cornea
Drainage
Sol-gels
Particle size
Anti-Bacterial Agents
In Vitro Techniques
Therapeutics

Keywords

  • Chitosan
  • CLSM
  • Histopathology
  • In situ gel
  • Levofloxacin
  • Nanoparticle

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Formulation and optimization of levofloxacin loaded chitosan nanoparticle for ocular delivery : In-vitro characterization, ocular tolerance and antibacterial activity. / Ameeduzzafar; Imam, Syed Sarim; Syed Nasir Abbas, Bukhari; Ahmad, Javed; Ali, Asgar.

In: International Journal of Biological Macromolecules, Vol. 108, 01.03.2018, p. 650-659.

Research output: Contribution to journalArticle

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