Folic acid targeted Mn

ZnS quantum dots for theranostic applications of cancer cell imaging and therapy

Ibrahim Birma Bwatanglang, Faruq Mohammad, Nor Azah Yusof, Jaafar Abdullah, Mohd Zobir Hussein, Noorjahan Banu Alitheen, Nadiah Abu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In this study, we synthesized a multifunctional nanoparticulate system with specific targeting, imaging, and drug delivering functionalities by following a three-step protocol that operates at room temperature and solely in aqueous media. The synthesis involves the encapsulation of luminescent Mn:ZnS quantum dots (QDs) with chitosan not only as a stabilizer in biological environment, but also to further provide active binding sites for the conjugation of other biomolecules. Folic acid was incorporated as targeting agent for the specific targeting of the nanocarrier toward the cells overexpressing folate receptors. Thus, the formed composite emits orange–red fluorescence around 600 nm and investigated to the highest intensity at Mn2+ doping concentration of 15 at.% and relatively more stable at low acidic and low alkaline pH levels. The structural characteristics and optical properties were thoroughly analyzed by using Fourier transform infrared, X-ray diffraction, dynamic light scattering, ultraviolet-visible, and fluorescence spectroscopy. Further characterization was conducted using thermogravimetric analysis, high-resolution transmission electron microscopy, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence, and X-ray photoelectron spectroscopy. The cell viability and proliferation studies by means of MTT assay have demonstrated that the as-synthesized composites do not exhibit any toxicity toward the human breast cell line MCF-10 (noncancer) and the breast cancer cell lines (MCF-7 and MDA-MB-231) up to a 500 μg/mL concentration. The cellular uptake of the nanocomposites was assayed by confocal laser scanning microscope by taking advantage of the conjugated Mn:ZnS QDs as fluorescence makers. The result showed that the functionalization of the chitosan-encapsulated QDs with folic acid enhanced the internalization and binding affinity of the nanocarrier toward folate receptor-overexpressed cells. Therefore, we hypothesized that due to the nontoxic nature of the composite, the as-synthesized nanoparticulate system can be used as a promising candidate for theranostic applications, especially for a simultaneous targeted drug delivery and cellular imaging.

Original languageEnglish
Pages (from-to)413-428
Number of pages16
JournalInternational Journal of Nanomedicine
Volume11
DOIs
Publication statusPublished - 22 Jan 2016
Externally publishedYes

Fingerprint

Quantum Dots
Cell- and Tissue-Based Therapy
Folic Acid
Semiconductor quantum dots
Fluorescence
Cells
Chitosan
Imaging techniques
Acids
Composite materials
Neoplasms
Ultraviolet visible spectroscopy
Fluorescence spectroscopy
Biomolecules
Infrared Rays
Dynamic light scattering
Binding sites
X-Ray Emission Spectrometry
High resolution transmission electron microscopy
Encapsulation

Keywords

  • Cancer diagnosis
  • Fluorescence imaging
  • Folic acid
  • Mn:ZnS
  • Quantum dots
  • Theranostics

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Folic acid targeted Mn : ZnS quantum dots for theranostic applications of cancer cell imaging and therapy. / Bwatanglang, Ibrahim Birma; Mohammad, Faruq; Yusof, Nor Azah; Abdullah, Jaafar; Hussein, Mohd Zobir; Alitheen, Noorjahan Banu; Abu, Nadiah.

In: International Journal of Nanomedicine, Vol. 11, 22.01.2016, p. 413-428.

Research output: Contribution to journalArticle

Bwatanglang, Ibrahim Birma ; Mohammad, Faruq ; Yusof, Nor Azah ; Abdullah, Jaafar ; Hussein, Mohd Zobir ; Alitheen, Noorjahan Banu ; Abu, Nadiah. / Folic acid targeted Mn : ZnS quantum dots for theranostic applications of cancer cell imaging and therapy. In: International Journal of Nanomedicine. 2016 ; Vol. 11. pp. 413-428.
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