Fluorescence-in-situ-hybridization in the surveillance of urothelial cancers: Can use of cystoscopy or ureteroscopy be deferred?

Chee Kong Christopher Ho, Wei Phin Tan, Rajadurai Pathmanathan, Wei Keith Tan, Hui Meng Tan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Fluorescence in situ hybridization (FISH) testing may be useful to screen for bladder carcinoma or dysplasia by detecting aneuploidy chromosomes 3, 7, 17 and deletion of the chromosome 9p21 locus in urine specimens. This study aimed to assess the sensitivity, specificity, positive and negative predictive value of FISH in a multi-ethnic population in Asia. Materials and Methods: Patients with haematuria and/or past history of urothelial cancer on follow-up had their voided urine tested with FISH. Patients then underwent cystoscopy/ureteroscopy and any lesions seen were biopsied. The histopathological reports of the bladder or ureteroscopic mucosal biopsies were then compared with the FISH test results. Results: Two hundred sixty patients were recruited. The sensitivity and specificity of the FISH test was 89.2% and 83.4% respectively. The positive (PPV) and negative predictive values (NPV) were 47.1% and 97.9%. By excluding patients who had positive deletion of chromosome 9, the overall results of the screening test improved: sensitivity 84.6%; specificity 96.4%; PPV 75.9% and NPV 97.9%. Conclusions: UroVysion FISH has a high specificity of detecting urothelial cancer or dysplasia when deletion of chromosome 9 is excluded. Negative UroVysion FISH-tests may allow us to conserve health resources and minimize trauma by deferring cystoscopic or ureteroscopic examination.

Original languageEnglish
Pages (from-to)4057-4059
Number of pages3
JournalAsian Pacific Journal of Cancer Prevention
Volume14
Issue number7
DOIs
Publication statusPublished - 2013

Fingerprint

Ureteroscopy
Cystoscopy
Fluorescence In Situ Hybridization
Neoplasms
Chromosomes, Human, Pair 9
Urinary Bladder
Urine
Sensitivity and Specificity
Chromosome Deletion
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Health Resources
Aneuploidy
Hematuria
Carcinoma
Biopsy
Wounds and Injuries

Keywords

  • Asia
  • Cancer
  • Fluorescence-in-situ-hybridization
  • Urothelial
  • UroVysion

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology

Cite this

Fluorescence-in-situ-hybridization in the surveillance of urothelial cancers : Can use of cystoscopy or ureteroscopy be deferred? / Ho, Chee Kong Christopher; Tan, Wei Phin; Pathmanathan, Rajadurai; Tan, Wei Keith; Tan, Hui Meng.

In: Asian Pacific Journal of Cancer Prevention, Vol. 14, No. 7, 2013, p. 4057-4059.

Research output: Contribution to journalArticle

Ho, Chee Kong Christopher ; Tan, Wei Phin ; Pathmanathan, Rajadurai ; Tan, Wei Keith ; Tan, Hui Meng. / Fluorescence-in-situ-hybridization in the surveillance of urothelial cancers : Can use of cystoscopy or ureteroscopy be deferred?. In: Asian Pacific Journal of Cancer Prevention. 2013 ; Vol. 14, No. 7. pp. 4057-4059.
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abstract = "Background: Fluorescence in situ hybridization (FISH) testing may be useful to screen for bladder carcinoma or dysplasia by detecting aneuploidy chromosomes 3, 7, 17 and deletion of the chromosome 9p21 locus in urine specimens. This study aimed to assess the sensitivity, specificity, positive and negative predictive value of FISH in a multi-ethnic population in Asia. Materials and Methods: Patients with haematuria and/or past history of urothelial cancer on follow-up had their voided urine tested with FISH. Patients then underwent cystoscopy/ureteroscopy and any lesions seen were biopsied. The histopathological reports of the bladder or ureteroscopic mucosal biopsies were then compared with the FISH test results. Results: Two hundred sixty patients were recruited. The sensitivity and specificity of the FISH test was 89.2{\%} and 83.4{\%} respectively. The positive (PPV) and negative predictive values (NPV) were 47.1{\%} and 97.9{\%}. By excluding patients who had positive deletion of chromosome 9, the overall results of the screening test improved: sensitivity 84.6{\%}; specificity 96.4{\%}; PPV 75.9{\%} and NPV 97.9{\%}. Conclusions: UroVysion FISH has a high specificity of detecting urothelial cancer or dysplasia when deletion of chromosome 9 is excluded. Negative UroVysion FISH-tests may allow us to conserve health resources and minimize trauma by deferring cystoscopic or ureteroscopic examination.",
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