Fluorescence in situ hybridization analysis using PAX8- and PPARG-specific probes reveals the presence of PAX8-PPARG translocation and 3p25 aneusomy in follicular thyroid neoplasms

Wai Kit Chia, Noor Akmal Sharifah, Reena Rahayu Md Zain, Zakaria Zubaidah, Ching Huat Clarence-Ko, Rohaizak Muhammad, Ibrahim Naqiyah, Srijit Das, Abdullah Nor Hisham, Arbi Asmiati, Md Kaslan Rafie

Research output: Contribution to journalArticle

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Abstract

At the present time, the differentiation between follicular thyroid carcinoma (FTC) and adenoma can be made only postoperatively and is based on the presence of capsular or vascular invasion. The ability to differentiate preoperatively between the malignant and benign forms of follicular thyroid tumors assumes greater importance in any clinical setting. The PAX8-PPARG translocation has been reported to occur in the majority of FTC. In this study, a group of 60 follicular thyroid neoplasms [18 FTC, 1 Hurthle cell carcinoma (HCC), 24 follicular thyroid adenomas (FTA), 5 Hurthle cell adenomas (HCA), and 12 follicular variants of papillary thyroid carcinomas (FV-PTC)] were analyzed to determine the prevalence of the PAX8-PPARG translocation by fluorescence in situ hybridization. The PAX8-PPARG translocation was detected in 2/18 FTC (11.1%). In addition, 2/18 (11.1%) FTC and 1/5 (20%) HCA showed 3p25 aneusomy only. The frequency of the translocation detected in the study was lower compared to the earlier studies conducted in Western countries. This might be attributed to the ethnic background and geographic location. Detection of either the PAX8-PPARG translocation or the 3p25 aneusomy in FTC indicates that these are independent genetic events. It is hereby concluded that 3p25 aneusomy or PAX8-PPARG translocation may play an important role in the molecular pathogenesis of follicular thyroid tumors.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume196
Issue number1
DOIs
Publication statusPublished - 1 Jan 2010

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Follicular Adenocarcinoma
Fluorescence In Situ Hybridization
Thyroid Neoplasms
Oxyphil Cells
Adenoma
Thyroid Gland
Geographic Locations
Blood Vessels
Neoplasms
Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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Fluorescence in situ hybridization analysis using PAX8- and PPARG-specific probes reveals the presence of PAX8-PPARG translocation and 3p25 aneusomy in follicular thyroid neoplasms. / Chia, Wai Kit; Sharifah, Noor Akmal; Md Zain, Reena Rahayu; Zubaidah, Zakaria; Clarence-Ko, Ching Huat; Muhammad, Rohaizak; Naqiyah, Ibrahim; Das, Srijit; Hisham, Abdullah Nor; Asmiati, Arbi; Rafie, Md Kaslan.

In: Cancer Genetics and Cytogenetics, Vol. 196, No. 1, 01.01.2010, p. 7-13.

Research output: Contribution to journalArticle

Chia, Wai Kit ; Sharifah, Noor Akmal ; Md Zain, Reena Rahayu ; Zubaidah, Zakaria ; Clarence-Ko, Ching Huat ; Muhammad, Rohaizak ; Naqiyah, Ibrahim ; Das, Srijit ; Hisham, Abdullah Nor ; Asmiati, Arbi ; Rafie, Md Kaslan. / Fluorescence in situ hybridization analysis using PAX8- and PPARG-specific probes reveals the presence of PAX8-PPARG translocation and 3p25 aneusomy in follicular thyroid neoplasms. In: Cancer Genetics and Cytogenetics. 2010 ; Vol. 196, No. 1. pp. 7-13.
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abstract = "At the present time, the differentiation between follicular thyroid carcinoma (FTC) and adenoma can be made only postoperatively and is based on the presence of capsular or vascular invasion. The ability to differentiate preoperatively between the malignant and benign forms of follicular thyroid tumors assumes greater importance in any clinical setting. The PAX8-PPARG translocation has been reported to occur in the majority of FTC. In this study, a group of 60 follicular thyroid neoplasms [18 FTC, 1 Hurthle cell carcinoma (HCC), 24 follicular thyroid adenomas (FTA), 5 Hurthle cell adenomas (HCA), and 12 follicular variants of papillary thyroid carcinomas (FV-PTC)] were analyzed to determine the prevalence of the PAX8-PPARG translocation by fluorescence in situ hybridization. The PAX8-PPARG translocation was detected in 2/18 FTC (11.1{\%}). In addition, 2/18 (11.1{\%}) FTC and 1/5 (20{\%}) HCA showed 3p25 aneusomy only. The frequency of the translocation detected in the study was lower compared to the earlier studies conducted in Western countries. This might be attributed to the ethnic background and geographic location. Detection of either the PAX8-PPARG translocation or the 3p25 aneusomy in FTC indicates that these are independent genetic events. It is hereby concluded that 3p25 aneusomy or PAX8-PPARG translocation may play an important role in the molecular pathogenesis of follicular thyroid tumors.",
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AU - Md Zain, Reena Rahayu

AU - Zubaidah, Zakaria

AU - Clarence-Ko, Ching Huat

AU - Muhammad, Rohaizak

AU - Naqiyah, Ibrahim

AU - Das, Srijit

AU - Hisham, Abdullah Nor

AU - Asmiati, Arbi

AU - Rafie, Md Kaslan

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