Fluorescence-based active site probes for profiling deubiquitinating enzymes

Joanna F. McGouran, Holger B. Kramer, Muhammad Mukram Mohamed Mackeen, Katalin Di Gleria, Mikael Altun, Benedikt M. Kessler

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.

Original languageEnglish
Pages (from-to)3379-3383
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume10
Issue number17
DOIs
Publication statusPublished - 7 May 2012
Externally publishedYes

Fingerprint

Optical Imaging
Ubiquitin
Cell Extracts
Fluorescent Dyes
enzymes
Catalytic Domain
Fluorescence
Gels
fluorescence
enzyme activity
probes
Enzyme activity
Enzymes
attachment
dyes
traps
gels
Imaging techniques
cells
Deubiquitinating Enzymes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Fluorescence-based active site probes for profiling deubiquitinating enzymes. / McGouran, Joanna F.; Kramer, Holger B.; Mohamed Mackeen, Muhammad Mukram; Di Gleria, Katalin; Altun, Mikael; Kessler, Benedikt M.

In: Organic and Biomolecular Chemistry, Vol. 10, No. 17, 07.05.2012, p. 3379-3383.

Research output: Contribution to journalArticle

McGouran, Joanna F. ; Kramer, Holger B. ; Mohamed Mackeen, Muhammad Mukram ; Di Gleria, Katalin ; Altun, Mikael ; Kessler, Benedikt M. / Fluorescence-based active site probes for profiling deubiquitinating enzymes. In: Organic and Biomolecular Chemistry. 2012 ; Vol. 10, No. 17. pp. 3379-3383.
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