Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity

Ochuko L. Erukainure, Osman Mesaik Mohammed Ahmed Hassan, Aliyu Muhammad, Chika I. Chukwuma, Neha Manhas, Parvesh Singh, Oluwole S. Aremu, Md Shahidul Islam

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The immunomodulatory potentials of the crude methanolic extract and fractions [n-hexane (Hex), n-dichloromethane (DCM), ethyl acetate (EtOAc) and n–butanol (BuOH)] of Clerodendrum volubile flowers were investigated on whole blood phagocytic oxidative burst using luminol-amplified chemiluminescence technique. They were also investigated for their free radicals scavenging activities. The DCM fraction showed significant (p < 0.05) anti-oxidative burst and free radical scavenging activities indicating high immunomodulatory and antioxidant potencies respectively. Cytotoxicity assay of the DCM fraction revealed a cytotoxic effect on CC-1 normal cell line. GCMS analysis revealed the presence of triacetin; 3,6-dimethyl-3-octanol; 2R – Acetoxymethyl-1,3,3–trimethtyl – 4 t – (3-methyl-2-buten-1-yl) – 1c – cyclohexanol and Stigmastan – 3,5–diene in DCM fraction. These compounds were docked with the active sites of cyclooxygenase-2 (COX-2). Triacetin, 3,6-dimethyl-3-Octanol, and 2R-Acetoxymethyl-1,3,3-trimethtyl-4t-(3-methyl-2-buten-1-yl)-1c-cyclohexanol docked comfortably with COX-2 with good scoring function (-CDocker energy) indicating their inhibitory potency against COX-2. 3,6–dimethyl-3-Octanol, displayed the lowest predicted free energy of binding (−21.4 kcal mol−1) suggesting its stronger interaction with COX-2, this was followed by 2R – Acetoxymethyl-1, 3, 3-trimethtyl-4t-(3-methyl-2-buten-1-yl)-1c-cyclhexanol (BE = −20.5 kcal mol−1), and triacetin (BE = −10.9 kcal mol−1). Stigmastan – 3,5–diene failed to dock with COX-2. The observed suppressive effect of the DCM fraction of C. volubile flower methanolic extract on phagocytic oxidative burst indicates an immunomodulatory potential. This is further reflected in its free scavenging activities and synergetic modulation of COX-2 activities by its identified compounds in silico.

Original languageEnglish
Pages (from-to)1478-1484
Number of pages7
JournalBiomedicine and Pharmacotherapy
Volume83
DOIs
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Fingerprint

Clerodendrum
Respiratory Burst
Immunomodulation
Cyclooxygenase 2
Methylene Chloride
Triacetin
Octanols
Cyclohexanols
Free Radicals
Luminol
Luminescence
Complex Mixtures
Computer Simulation
Gas Chromatography-Mass Spectrometry
Catalytic Domain
Antioxidants
Cell Line

Keywords

  • Antiinflamation
  • Antioxidant
  • Immunomodulation
  • Oxidative burst

ASJC Scopus subject areas

  • Pharmacology

Cite this

Erukainure, O. L., Mohammed Ahmed Hassan, O. M., Muhammad, A., Chukwuma, C. I., Manhas, N., Singh, P., ... Islam, M. S. (2016). Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity. Biomedicine and Pharmacotherapy, 83, 1478-1484. https://doi.org/10.1016/j.biopha.2016.09.002

Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity. / Erukainure, Ochuko L.; Mohammed Ahmed Hassan, Osman Mesaik; Muhammad, Aliyu; Chukwuma, Chika I.; Manhas, Neha; Singh, Parvesh; Aremu, Oluwole S.; Islam, Md Shahidul.

In: Biomedicine and Pharmacotherapy, Vol. 83, 01.10.2016, p. 1478-1484.

Research output: Contribution to journalArticle

Erukainure, OL, Mohammed Ahmed Hassan, OM, Muhammad, A, Chukwuma, CI, Manhas, N, Singh, P, Aremu, OS & Islam, MS 2016, 'Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity', Biomedicine and Pharmacotherapy, vol. 83, pp. 1478-1484. https://doi.org/10.1016/j.biopha.2016.09.002
Erukainure, Ochuko L. ; Mohammed Ahmed Hassan, Osman Mesaik ; Muhammad, Aliyu ; Chukwuma, Chika I. ; Manhas, Neha ; Singh, Parvesh ; Aremu, Oluwole S. ; Islam, Md Shahidul. / Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity. In: Biomedicine and Pharmacotherapy. 2016 ; Vol. 83. pp. 1478-1484.
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KW - Antioxidant

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