Flow cytometric analysis of intracellular myeloperoxidase distinguishes lymphocytes, monocytes and granulocytes.

S. P. Tay, S. K. Cheong, Noor Hamidah Hussin, O. Ainoon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A study was undertaken to evaluate the ability of flow cytometric analysis of intracellular myeloperoxidase (MPO) in differentiating populations of lymphocytes (L), monocytes (M) and granulocytes (G), by means of lysed whole blood method. Anticoagulated blood from 23 normal individuals was lysed with FACS lysing solution and permeabilized with FACS permeabilizing solution before subjected to direct immunofluorescence staining. The geometric means of the fluorescence intensity were measured using FACSCalibur flow cytometer (Becton Dickinson). Populations of L, M and G were gated based on their light scatter characteristics and expression of CD14 and CD45. Then, the fluorescence intensity of MPO expression was studied in these individual cell populations. The results showed that fluorescence intensity of MPO was the strongest in G and weakest in L, whereas M showed intermediate fluorescence intensity. Our findings reveal that discrimination of these three cell types is achievable based upon the sole expression of intracellular MPO.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalThe Malaysian journal of pathology
Volume20
Issue number2
Publication statusPublished - 1998

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Granulocytes
Peroxidase
Monocytes
Fluorescence
Lymphocytes
Population
Direct Fluorescent Antibody Technique
Staining and Labeling
Light

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Flow cytometric analysis of intracellular myeloperoxidase distinguishes lymphocytes, monocytes and granulocytes. / Tay, S. P.; Cheong, S. K.; Hussin, Noor Hamidah; Ainoon, O.

In: The Malaysian journal of pathology, Vol. 20, No. 2, 1998, p. 91-94.

Research output: Contribution to journalArticle

Tay, S. P. ; Cheong, S. K. ; Hussin, Noor Hamidah ; Ainoon, O. / Flow cytometric analysis of intracellular myeloperoxidase distinguishes lymphocytes, monocytes and granulocytes. In: The Malaysian journal of pathology. 1998 ; Vol. 20, No. 2. pp. 91-94.
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