Flavonoids of Piper sarmentosum and its cytoprotective effects against oxidative stress

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Abnormalities in endothelial cell structure and function may lead to diseases such as thrombosis and atherosclerosis. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases including atherosclerosis. Previous studies have shown a relationship between a diet rich in flavonoid and a reduced incidence of cardiovascular diseases. Piper sarmentosum (PS) is a plant with high flavonoid content and it possesses antioxidant and anti-atherosclerotic activities. Therefore this study aimed to investigate the flavonoids present in aqueous extract of PS (AEPS) and its cytoprotective effects in oxidative stress-induced human umbilical vein endothelial cells (HUVEC). AEPS contained high total phenolic content (91.02 ± 0.02 mg QE/g DM) and total flavonoid content (48.57 ± 0.03 mg GAE/g DM). Screening using high performance liquid chromatography (HPLC) technique showed the presence of rutin and vitexin as the main flavonoids in AEPS. HUVEC were exposed to 180 μM H2O2 and treated with various concentrations of rutin or vitexin (10 to 400 μM) for 24 hours. Both rutin and vitexin at the concentration of 150-400 μM significantly increased the viability of H2O2-induced HUVEC as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Therefore rutin and vitexin as the main flavonoids present in PS may be involved in the protective effects of PS against oxidative stress.

Original languageEnglish
Pages (from-to)705-714
Number of pages10
JournalEXCLI Journal
Volume11
Publication statusPublished - 9 Nov 2012

Fingerprint

Piper
Flavonoids
Rutin
Oxidative Stress
oxidative stress
flavonoids
rutin
Human Umbilical Vein Endothelial Cells
atherosclerosis
cardiovascular diseases
Atherosclerosis
extracts
Cardiovascular Diseases
water
thrombosis
cell structures
bromides
Bromides
endothelial cells
protective effect

Keywords

  • Flavonoid
  • Human umbilical vein endothelial cells
  • Oxidative stress
  • Piper sarmentosum
  • Rutin
  • Vitexin

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Animal Science and Zoology

Cite this

Flavonoids of Piper sarmentosum and its cytoprotective effects against oxidative stress. / Ugusman, Azizah; Zakaria, Zaiton; Kien Hui, Chua; Nordin, Nor Anita Megat Mohd; Abdullah Mahdy, Zaleha.

In: EXCLI Journal, Vol. 11, 09.11.2012, p. 705-714.

Research output: Contribution to journalArticle

@article{03985194fa184b45b5f64757bda46070,
title = "Flavonoids of Piper sarmentosum and its cytoprotective effects against oxidative stress",
abstract = "Abnormalities in endothelial cell structure and function may lead to diseases such as thrombosis and atherosclerosis. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases including atherosclerosis. Previous studies have shown a relationship between a diet rich in flavonoid and a reduced incidence of cardiovascular diseases. Piper sarmentosum (PS) is a plant with high flavonoid content and it possesses antioxidant and anti-atherosclerotic activities. Therefore this study aimed to investigate the flavonoids present in aqueous extract of PS (AEPS) and its cytoprotective effects in oxidative stress-induced human umbilical vein endothelial cells (HUVEC). AEPS contained high total phenolic content (91.02 ± 0.02 mg QE/g DM) and total flavonoid content (48.57 ± 0.03 mg GAE/g DM). Screening using high performance liquid chromatography (HPLC) technique showed the presence of rutin and vitexin as the main flavonoids in AEPS. HUVEC were exposed to 180 μM H2O2 and treated with various concentrations of rutin or vitexin (10 to 400 μM) for 24 hours. Both rutin and vitexin at the concentration of 150-400 μM significantly increased the viability of H2O2-induced HUVEC as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Therefore rutin and vitexin as the main flavonoids present in PS may be involved in the protective effects of PS against oxidative stress.",
keywords = "Flavonoid, Human umbilical vein endothelial cells, Oxidative stress, Piper sarmentosum, Rutin, Vitexin",
author = "Azizah Ugusman and Zaiton Zakaria and {Kien Hui}, Chua and Nordin, {Nor Anita Megat Mohd} and {Abdullah Mahdy}, Zaleha",
year = "2012",
month = "11",
day = "9",
language = "English",
volume = "11",
pages = "705--714",
journal = "EXCLI Journal",
issn = "1611-2156",
publisher = "University of Mainz",

}

TY - JOUR

T1 - Flavonoids of Piper sarmentosum and its cytoprotective effects against oxidative stress

AU - Ugusman, Azizah

AU - Zakaria, Zaiton

AU - Kien Hui, Chua

AU - Nordin, Nor Anita Megat Mohd

AU - Abdullah Mahdy, Zaleha

PY - 2012/11/9

Y1 - 2012/11/9

N2 - Abnormalities in endothelial cell structure and function may lead to diseases such as thrombosis and atherosclerosis. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases including atherosclerosis. Previous studies have shown a relationship between a diet rich in flavonoid and a reduced incidence of cardiovascular diseases. Piper sarmentosum (PS) is a plant with high flavonoid content and it possesses antioxidant and anti-atherosclerotic activities. Therefore this study aimed to investigate the flavonoids present in aqueous extract of PS (AEPS) and its cytoprotective effects in oxidative stress-induced human umbilical vein endothelial cells (HUVEC). AEPS contained high total phenolic content (91.02 ± 0.02 mg QE/g DM) and total flavonoid content (48.57 ± 0.03 mg GAE/g DM). Screening using high performance liquid chromatography (HPLC) technique showed the presence of rutin and vitexin as the main flavonoids in AEPS. HUVEC were exposed to 180 μM H2O2 and treated with various concentrations of rutin or vitexin (10 to 400 μM) for 24 hours. Both rutin and vitexin at the concentration of 150-400 μM significantly increased the viability of H2O2-induced HUVEC as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Therefore rutin and vitexin as the main flavonoids present in PS may be involved in the protective effects of PS against oxidative stress.

AB - Abnormalities in endothelial cell structure and function may lead to diseases such as thrombosis and atherosclerosis. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases including atherosclerosis. Previous studies have shown a relationship between a diet rich in flavonoid and a reduced incidence of cardiovascular diseases. Piper sarmentosum (PS) is a plant with high flavonoid content and it possesses antioxidant and anti-atherosclerotic activities. Therefore this study aimed to investigate the flavonoids present in aqueous extract of PS (AEPS) and its cytoprotective effects in oxidative stress-induced human umbilical vein endothelial cells (HUVEC). AEPS contained high total phenolic content (91.02 ± 0.02 mg QE/g DM) and total flavonoid content (48.57 ± 0.03 mg GAE/g DM). Screening using high performance liquid chromatography (HPLC) technique showed the presence of rutin and vitexin as the main flavonoids in AEPS. HUVEC were exposed to 180 μM H2O2 and treated with various concentrations of rutin or vitexin (10 to 400 μM) for 24 hours. Both rutin and vitexin at the concentration of 150-400 μM significantly increased the viability of H2O2-induced HUVEC as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Therefore rutin and vitexin as the main flavonoids present in PS may be involved in the protective effects of PS against oxidative stress.

KW - Flavonoid

KW - Human umbilical vein endothelial cells

KW - Oxidative stress

KW - Piper sarmentosum

KW - Rutin

KW - Vitexin

UR - http://www.scopus.com/inward/record.url?scp=84872952907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872952907&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 705

EP - 714

JO - EXCLI Journal

JF - EXCLI Journal

SN - 1611-2156

ER -