Flavonoids of Artocarpus heterophyllus Lam. heartwood inhibit the innate immune responses of human phagocytes

Abdi Wira Septama, Ibrahim Jantan, Pharkphoom Panichayupakaranant

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: To investigate the effects of flavonoids isolated from Artocarpus heterophyllus. heartwood on chemotaxis, phagocytosis, reactive oxygen species (ROS) production and myeloperoxidase (MPO) activity of human phagocytes. Methods: Chemotaxis was evaluated using a modified Boyden chamber and phagocytosis was determined by flowcytometer. Respiratory burst was investigated by luminol-based chemiluminescence assay while MPO activity was determined by colorimetric assay. Key findings: Artocarpanone and artocarpin strongly inhibited all steps of phagocytosis. Artocarpanone and artocarpin showed strong chemotactic activity with IC50 values of 6.96 and 6.10 μm, respectively, which were lower than that of ibuprofen (7.37 μm). Artocarpanone was the most potent compound in inhibiting ROS production of polymorphonuclear leucocytes and monocytes with IC50 values comparable to those of aspirin. Artocarpin at 100 μg/ml inhibited phagocytosis of opsonized bacteria (28.3%). It also strongly inhibited MPO release with an IC50 value (23.3 μm) lower than that of indomethacin (69 μm). Structure–activity analysis indicated that the number of hydroxyl group, the presence of prenyl group and variation of C-2 and C-3 bonds might contribute towards their phagocytosis. Conclusions: Artocarpanone and artocarpin were able to suppress strongly the phagocytosis of human phagocytes at different steps and have potential to be developed into potent anti-inflammatory agents.

Original languageEnglish
Pages (from-to)1242-1252
Number of pages11
JournalJournal of Pharmacy and Pharmacology
Volume70
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018

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Artocarpus
Phagocytes
Phagocytosis
Flavonoids
Innate Immunity
Peroxidase
Inhibitory Concentration 50
Chemotaxis
Reactive Oxygen Species
Luminol
Respiratory Burst
Ibuprofen
Luminescence
Human Activities
Indomethacin
Hydroxyl Radical
Aspirin
Monocytes
Neutrophils
Anti-Inflammatory Agents

Keywords

  • artocarpanone
  • artocarpin
  • Artocarpus heterophyllus
  • immunosuppression
  • phagocytosis

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Flavonoids of Artocarpus heterophyllus Lam. heartwood inhibit the innate immune responses of human phagocytes. / Septama, Abdi Wira; Jantan, Ibrahim; Panichayupakaranant, Pharkphoom.

In: Journal of Pharmacy and Pharmacology, Vol. 70, No. 9, 01.09.2018, p. 1242-1252.

Research output: Contribution to journalArticle

Septama, Abdi Wira ; Jantan, Ibrahim ; Panichayupakaranant, Pharkphoom. / Flavonoids of Artocarpus heterophyllus Lam. heartwood inhibit the innate immune responses of human phagocytes. In: Journal of Pharmacy and Pharmacology. 2018 ; Vol. 70, No. 9. pp. 1242-1252.
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