Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells

Md Mahmudul Hasan, Qamar Uddin Ahmed, Siti Zaiton Mat Soad, Jalifah Latip, Muhammad Taher, Tengku Muhamad Faris Syafiq, Murni Nazira Sarian, Alhassan Muhammad Alhassan, Zainul Amiruddin Zakaria

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Tetracera indica Merr. (Family: Dilleniaceae), known to the Malay as 'Mempelas paya', is one of the medicinal plants used in the treatment of diabetes in Malaysia. However, no proper scientific study has been carried out to verify the traditional claim of T. indica as an antidiabetic agent. Hence, the aims of the present study were to determine the in vitro antidiabetic potential of the T. indica stems ethanol extract, subfractions and isolated compounds. Methods: The ethanol extract and its subfractions, and isolated compounds from T. indica stems were subjected to cytotoxicity test using MTT viability assay on 3T3-L1 pre-adipocytes. Then, the test groups were subjected to the in vitro antidiabetic investigation using 3T3-L1 pre-adipocytes and differentiated adipocytes to determine the insulin-like and insulin sensitizing activities. Rosiglitazone was used as a standard antidiabetic agent. All compounds were also subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. Test solutions were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Isolation of bioactive compounds from the most effective subfraction (ethyl acetate) was performed through repeated silica gel and sephadex LH-20 column chromatographies and their structures were elucidated through 1H-and 13C-NMR spectroscopy. Results: Four monoflavonoids, namely, wogonin, norwogonin, quercetin and techtochrysin were isolated from the T. indica stems ethanol extract. Wogonin, norwogonin and techtochrysin induced significant (P < 0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Wogonin and norwogonin also exhibited significant (P < 0.05) glucose uptake activity. Conclusion: The present study demonstrated that the flavonoids isolated from the T. indica stems possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds. This also rationalizes the traditional use of T. indica in the management of diabetes in Malaysia.

Original languageEnglish
Article number431
JournalBMC Complementary and Alternative Medicine
Volume17
Issue number1
DOIs
Publication statusPublished - 30 Aug 2017

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3T3-L1 Cells
Adipogenesis
Flavonoids
Adipocytes
Hypoglycemic Agents
rosiglitazone
Insulin
Glucose
Ethanol
Malaysia
Dilleniaceae
Silica Gel
Quercetin
Medicinal Plants
Chromatography
Magnetic Resonance Spectroscopy
Fluorescence
norwogonin
wogonin

Keywords

  • 2-NBDG glucose uptake activity
  • 3T3-L1 preadipocyte cells
  • Adipogenesis
  • Flavonoids
  • Insulin like activity
  • Insulin sensitizing activity
  • Tetracera indica Merr.

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells. / Hasan, Md Mahmudul; Ahmed, Qamar Uddin; Soad, Siti Zaiton Mat; Latip, Jalifah; Taher, Muhammad; Syafiq, Tengku Muhamad Faris; Sarian, Murni Nazira; Alhassan, Alhassan Muhammad; Zakaria, Zainul Amiruddin.

In: BMC Complementary and Alternative Medicine, Vol. 17, No. 1, 431, 30.08.2017.

Research output: Contribution to journalArticle

Hasan, Md Mahmudul ; Ahmed, Qamar Uddin ; Soad, Siti Zaiton Mat ; Latip, Jalifah ; Taher, Muhammad ; Syafiq, Tengku Muhamad Faris ; Sarian, Murni Nazira ; Alhassan, Alhassan Muhammad ; Zakaria, Zainul Amiruddin. / Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells. In: BMC Complementary and Alternative Medicine. 2017 ; Vol. 17, No. 1.
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T1 - Flavonoids from Tetracera indica Merr. induce adipogenesis and exert glucose uptake activities in 3T3-L1 adipocyte cells

AU - Hasan, Md Mahmudul

AU - Ahmed, Qamar Uddin

AU - Soad, Siti Zaiton Mat

AU - Latip, Jalifah

AU - Taher, Muhammad

AU - Syafiq, Tengku Muhamad Faris

AU - Sarian, Murni Nazira

AU - Alhassan, Alhassan Muhammad

AU - Zakaria, Zainul Amiruddin

PY - 2017/8/30

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N2 - Background: Tetracera indica Merr. (Family: Dilleniaceae), known to the Malay as 'Mempelas paya', is one of the medicinal plants used in the treatment of diabetes in Malaysia. However, no proper scientific study has been carried out to verify the traditional claim of T. indica as an antidiabetic agent. Hence, the aims of the present study were to determine the in vitro antidiabetic potential of the T. indica stems ethanol extract, subfractions and isolated compounds. Methods: The ethanol extract and its subfractions, and isolated compounds from T. indica stems were subjected to cytotoxicity test using MTT viability assay on 3T3-L1 pre-adipocytes. Then, the test groups were subjected to the in vitro antidiabetic investigation using 3T3-L1 pre-adipocytes and differentiated adipocytes to determine the insulin-like and insulin sensitizing activities. Rosiglitazone was used as a standard antidiabetic agent. All compounds were also subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. Test solutions were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Isolation of bioactive compounds from the most effective subfraction (ethyl acetate) was performed through repeated silica gel and sephadex LH-20 column chromatographies and their structures were elucidated through 1H-and 13C-NMR spectroscopy. Results: Four monoflavonoids, namely, wogonin, norwogonin, quercetin and techtochrysin were isolated from the T. indica stems ethanol extract. Wogonin, norwogonin and techtochrysin induced significant (P < 0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Wogonin and norwogonin also exhibited significant (P < 0.05) glucose uptake activity. Conclusion: The present study demonstrated that the flavonoids isolated from the T. indica stems possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds. This also rationalizes the traditional use of T. indica in the management of diabetes in Malaysia.

AB - Background: Tetracera indica Merr. (Family: Dilleniaceae), known to the Malay as 'Mempelas paya', is one of the medicinal plants used in the treatment of diabetes in Malaysia. However, no proper scientific study has been carried out to verify the traditional claim of T. indica as an antidiabetic agent. Hence, the aims of the present study were to determine the in vitro antidiabetic potential of the T. indica stems ethanol extract, subfractions and isolated compounds. Methods: The ethanol extract and its subfractions, and isolated compounds from T. indica stems were subjected to cytotoxicity test using MTT viability assay on 3T3-L1 pre-adipocytes. Then, the test groups were subjected to the in vitro antidiabetic investigation using 3T3-L1 pre-adipocytes and differentiated adipocytes to determine the insulin-like and insulin sensitizing activities. Rosiglitazone was used as a standard antidiabetic agent. All compounds were also subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. Test solutions were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Isolation of bioactive compounds from the most effective subfraction (ethyl acetate) was performed through repeated silica gel and sephadex LH-20 column chromatographies and their structures were elucidated through 1H-and 13C-NMR spectroscopy. Results: Four monoflavonoids, namely, wogonin, norwogonin, quercetin and techtochrysin were isolated from the T. indica stems ethanol extract. Wogonin, norwogonin and techtochrysin induced significant (P < 0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Wogonin and norwogonin also exhibited significant (P < 0.05) glucose uptake activity. Conclusion: The present study demonstrated that the flavonoids isolated from the T. indica stems possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds. This also rationalizes the traditional use of T. indica in the management of diabetes in Malaysia.

KW - 2-NBDG glucose uptake activity

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KW - Adipogenesis

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KW - Insulin like activity

KW - Insulin sensitizing activity

KW - Tetracera indica Merr.

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