FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms

Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, Wai Kit Chia

    Research output: Chapter in Book/Report/Conference proceedingChapter

    3 Citations (Scopus)

    Abstract

    Fluorescence in situ hybridization (FISH) is increasingly gaining importance in clinical diagnostics settings. Due to the ability of the technique to detect chromosomal abnormalities in samples with low cellularity or containing a mixed population of cells even at a single-cell level, it has become more popular in cancer research and diagnosis. Here, we describe the FISH technique for detection of PAX8-PPARγ translocation in follicular thyroid neoplasms, and the optimal protocol for the detection of this fusion gene using in archival formalin-fixed paraffin-embedded (FFPE) thyroid tissue sections.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    Pages187-196
    Number of pages10
    Volume952
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    Volume952
    ISSN (Print)10643745

    Fingerprint

    Peroxisome Proliferator-Activated Receptors
    Fluorescence In Situ Hybridization
    Thyroid Neoplasms
    Gene Fusion
    Chromosome Aberrations
    Paraffin
    Formaldehyde
    Thyroid Gland
    Research
    Population
    Neoplasms

    Keywords

    • Fluorescence in situ hybridization (FISH)
    • Follicular thyroid neoplasms
    • Formalin-fixed paraffin-embedded (FFPE) tissue
    • PAX8-PPARγ fusion gene

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Cite this

    Sharifah, N. A. S. H., Zakaria, Z., & Chia, W. K. (2013). FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms. In Methods in Molecular Biology (Vol. 952, pp. 187-196). (Methods in Molecular Biology; Vol. 952). https://doi.org/10.1007/978-1-62703-155-4_13

    FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms. / Sharifah, Noor Akmal Syed Husain; Zakaria, Zubaidah; Chia, Wai Kit.

    Methods in Molecular Biology. Vol. 952 2013. p. 187-196 (Methods in Molecular Biology; Vol. 952).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Sharifah, NASH, Zakaria, Z & Chia, WK 2013, FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms. in Methods in Molecular Biology. vol. 952, Methods in Molecular Biology, vol. 952, pp. 187-196. https://doi.org/10.1007/978-1-62703-155-4_13
    Sharifah NASH, Zakaria Z, Chia WK. FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms. In Methods in Molecular Biology. Vol. 952. 2013. p. 187-196. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-155-4_13
    Sharifah, Noor Akmal Syed Husain ; Zakaria, Zubaidah ; Chia, Wai Kit. / FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms. Methods in Molecular Biology. Vol. 952 2013. pp. 187-196 (Methods in Molecular Biology).
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