First report on the molecular epidemiology of Malaysian Staphylococcus epidermidis isolated from a university teaching hospital

Nurul Azirah Mohamad Sani, Hassriana Fazilla Sapri, Hui Min Neoh, Salasawati Hussin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Staphylococcus epidermidis is a pathogen associated with nosocomial infections whose medical importance has increased due to progressively invasive medical procedures. In this study, we characterized the molecular epidemiology of S. epidermidis strains circulating in our university hospital situated in Kuala Lumpur, Malaysia.

Findings: A total of 798 S. epidermidis were isolated from our university hospital, where 56.3% of the isolates were found to be cefoxitin (methicillin) resistant and also positive for the mecA gene. Staphylococcus Cassette Chromosome mec (SCCmec) typing revealed that 39.6% of the methicillin-resistant S. epidermidis (MRSE) were SCCmec-untypeable, with 54.6% harboring the cassette chromosome recombinase C (ccrC) gene. A total of 67 isolates from the neonatal intensive care unit (NICU) was selected for pulsed-field gel electrophoresis (PFGE) typing, where 13 pulsotypes were identified at a cut-off value of 80% similarity. No significant association was found between the PFGE pulsotypes, SCCmec types and antibiotic susceptibilities. Polymerase chain reaction (PCR) assays to detect biofilm-associated genes in the ica operon and also 4 staphylococcal toxin genes (cna, seh, PVL genes and tst-1) revealed that only 8.0% isolates had the complete operon, while cna was the most prevalent toxin gene detected amongst the isolates (35.8%).

Conclusion: To our knowledge, this is the first report on the molecular epidemiology of Malaysian S. epidermidis. We found the strains to be low in virulence potential; nevertheless further studies have to be conducted to determine if this phenomenon translates into a better clinical outcome for patients.

Original languageEnglish
Article number597
JournalBMC Research Notes
Volume7
Issue number1
DOIs
Publication statusPublished - 3 Sep 2014

Fingerprint

Staphylococcus epidermidis
Molecular Epidemiology
Teaching Hospitals
Teaching
Genes
Chromosomes
Staphylococcus
Methicillin Resistance
Methicillin
Pulsed Field Gel Electrophoresis
Operon
Electrophoresis
Gels
Chromosomes, Human, 6-12 and X
Cefoxitin
Intensive care units
Recombinases
Neonatal Intensive Care Units
Polymerase chain reaction
Malaysia

Keywords

  • Ica operon characterization
  • Malaysian S. epidermidis
  • SCCmec typing
  • Virulence gene typing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

First report on the molecular epidemiology of Malaysian Staphylococcus epidermidis isolated from a university teaching hospital. / Sani, Nurul Azirah Mohamad; Sapri, Hassriana Fazilla; Neoh, Hui Min; Hussin, Salasawati.

In: BMC Research Notes, Vol. 7, No. 1, 597, 03.09.2014.

Research output: Contribution to journalArticle

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abstract = "Background: Staphylococcus epidermidis is a pathogen associated with nosocomial infections whose medical importance has increased due to progressively invasive medical procedures. In this study, we characterized the molecular epidemiology of S. epidermidis strains circulating in our university hospital situated in Kuala Lumpur, Malaysia.Findings: A total of 798 S. epidermidis were isolated from our university hospital, where 56.3{\%} of the isolates were found to be cefoxitin (methicillin) resistant and also positive for the mecA gene. Staphylococcus Cassette Chromosome mec (SCCmec) typing revealed that 39.6{\%} of the methicillin-resistant S. epidermidis (MRSE) were SCCmec-untypeable, with 54.6{\%} harboring the cassette chromosome recombinase C (ccrC) gene. A total of 67 isolates from the neonatal intensive care unit (NICU) was selected for pulsed-field gel electrophoresis (PFGE) typing, where 13 pulsotypes were identified at a cut-off value of 80{\%} similarity. No significant association was found between the PFGE pulsotypes, SCCmec types and antibiotic susceptibilities. Polymerase chain reaction (PCR) assays to detect biofilm-associated genes in the ica operon and also 4 staphylococcal toxin genes (cna, seh, PVL genes and tst-1) revealed that only 8.0{\%} isolates had the complete operon, while cna was the most prevalent toxin gene detected amongst the isolates (35.8{\%}).Conclusion: To our knowledge, this is the first report on the molecular epidemiology of Malaysian S. epidermidis. We found the strains to be low in virulence potential; nevertheless further studies have to be conducted to determine if this phenomenon translates into a better clinical outcome for patients.",
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