First molecular identification of Entamoeba moshkovskii in Malaysia

Tengku Shahrul Anuar, Hesham M. Al-Mekhlafi, Mohamed Kamel Abdul Ghani, Siti Nor Azreen, Fatmah Md Salleh, Nuraffini Ghazali, Mekadina Bernadus, Norhayati Moktar

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Entamoeba moshkovskii and Entamoeba dispar are microscopically indistinguishable from the pathogenic species Entamoeba histolytica. Although sporadic cases of human infection with E. moshkovskii have been reported, the amoeba is still considered primarily as a free-living amoeba. A cross-sectional study was carried out among Orang Asli communities in 3 different states of Peninsular Malaysia. Fecal samples were examined by formalin-ether sedimentation and trichrome staining techniques and then single-round PCR assay was used to detect E. moshkovskii. Out of 500 fecal samples examined microscopically, 93 (18·6%) samples were positive for E. histolytica/E. dispar/E. moshkovskii complex cysts and/or trophozoites. PCR products were detected in 106 fecal samples. E. moshkovskii isolates were detected in 13 (12·3%) fecal samples. Of the 13 E. moshkovskii-positive samples, 5 were of single isolation of E. moshkovskii, 6 were also positive for E. dispar, and only 2 samples were positive for E. dispar and E. histolytica. Moreover, 3 E. moshkovskii-positive samples were collected from symptomatic individuals while the remaining 10 samples were from asymptomatic subjects. This is the first report on the identification of E. moshkovskii in Malaysia. Further studies are needed to confirm the pathogenicity of E. moshkovskii infection and determine the epidemiology among Orang Asli communities in Malaysia. ©

Original languageEnglish
Pages (from-to)1521-1525
Number of pages5
JournalParasitology
Volume139
Issue number12
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Entamoeba
Entamoeba histolytica
Malaysia
Amoeba
Polymerase Chain Reaction
Trophozoites
Infection
sampling
Ether
Formaldehyde
Virulence
Cysts
Epidemiology
Cross-Sectional Studies
Staining and Labeling
trophozoites
formalin
cross-sectional studies
infection
epidemiology

Keywords

  • Entamoeba moshkovskii
  • Malaysia
  • Orang Asli
  • single-round PCR

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Anuar, T. S., Al-Mekhlafi, H. M., Abdul Ghani, M. K., Azreen, S. N., Md Salleh, F., Ghazali, N., ... Moktar, N. (2012). First molecular identification of Entamoeba moshkovskii in Malaysia. Parasitology, 139(12), 1521-1525. https://doi.org/10.1017/S0031182012001485

First molecular identification of Entamoeba moshkovskii in Malaysia. / Anuar, Tengku Shahrul; Al-Mekhlafi, Hesham M.; Abdul Ghani, Mohamed Kamel; Azreen, Siti Nor; Md Salleh, Fatmah; Ghazali, Nuraffini; Bernadus, Mekadina; Moktar, Norhayati.

In: Parasitology, Vol. 139, No. 12, 10.2012, p. 1521-1525.

Research output: Contribution to journalArticle

Anuar, TS, Al-Mekhlafi, HM, Abdul Ghani, MK, Azreen, SN, Md Salleh, F, Ghazali, N, Bernadus, M & Moktar, N 2012, 'First molecular identification of Entamoeba moshkovskii in Malaysia', Parasitology, vol. 139, no. 12, pp. 1521-1525. https://doi.org/10.1017/S0031182012001485
Anuar TS, Al-Mekhlafi HM, Abdul Ghani MK, Azreen SN, Md Salleh F, Ghazali N et al. First molecular identification of Entamoeba moshkovskii in Malaysia. Parasitology. 2012 Oct;139(12):1521-1525. https://doi.org/10.1017/S0031182012001485
Anuar, Tengku Shahrul ; Al-Mekhlafi, Hesham M. ; Abdul Ghani, Mohamed Kamel ; Azreen, Siti Nor ; Md Salleh, Fatmah ; Ghazali, Nuraffini ; Bernadus, Mekadina ; Moktar, Norhayati. / First molecular identification of Entamoeba moshkovskii in Malaysia. In: Parasitology. 2012 ; Vol. 139, No. 12. pp. 1521-1525.
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