Fine mapping of a grain weight quantitative trait locus, qGW6, Using near isogenic lines derived from oryza rufipogon IRGC105491 and oryza sativa cultivar MR219

M. S. Ngu, M. J. Thomson, M. A R Bhuiyan, C. Ho, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Grain weight is a major component of rice grain yield and is controlled by quantitative trait loci. Previously, a rice grain weight quantitative trait locus (qGW6) was detected near marker RM587 on chromosome 6 in a backcross population (BC2F2) derived from a cross between Oryza rufipogon IRGC105491 and O. sativa cv. MR219. Using a BC2F5 population, qGW6 was validated and mapped to a region of 4.8 cM (1.2 Mb) in the interval between RM508 and RM588. Fine mapping using a series of BC4F3 near isogenic lines further narrowed the interval containing qGW6 to 88 kb between markers RM19268 and RM19271.1. According to the Duncan multiple range test, 8 BC4F4 near isogenic lines had significantly higher 100-grain weight (4.8 to 7.5% over MR219) than their recurrent parent, MR219 (P < 0.05). According to the rice genome automated annotation database, there are 20 predicted genes in the 88-kb target region, and 9 of them have known functions. Among the genes with known functions in the target region, in silico gene expression analysis showed that 9 were differentially expressed during the seed development stage(s) from gene expression series GSE6893; however, only 3 of them have known functions. These candidates provide targets for further characterization of qGW6, which will assist in understanding the genetic control of grain weight in rice.

Original languageEnglish
Pages (from-to)9477-9488
Number of pages12
JournalGenetics and Molecular Research
Volume13
Issue number4
DOIs
Publication statusPublished - 11 Nov 2014

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Quantitative Trait Loci
Weights and Measures
Gene Expression
Chromosomes, Human, Pair 6
Computer Simulation
Population
Genes
Oryza
Seeds
Genome
Databases

Keywords

  • Fine mapping
  • Grain weight
  • Near isogenic lines
  • Oryza rufipogon
  • Quantitative trait locus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Fine mapping of a grain weight quantitative trait locus, qGW6, Using near isogenic lines derived from oryza rufipogon IRGC105491 and oryza sativa cultivar MR219. / Ngu, M. S.; Thomson, M. J.; Bhuiyan, M. A R; Ho, C.; V Ratnam, R Wickneswari.

In: Genetics and Molecular Research, Vol. 13, No. 4, 11.11.2014, p. 9477-9488.

Research output: Contribution to journalArticle

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