Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria

Imad Faik, Segun I. Oyedeji, Zulkarnain Md Idris, Iara J. de Messias-Reason, Bertrand Lell, Peter G. Kremsner, Jürgen F J Kun

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Human ficolin-2 (L-ficolin; FCN2) is a serum protein binding to sugar moieties of different human micro-pathogens forcing phagocytosis. Here, we investigate the clinical significance of FCN2 in African children with either mild or severe malaria (n = 130 and n = 108, respectively) from Gabon by analyzing three promoter SNPs (-986G>A, -602G>A, and -4A>G) and one single nucleotide polymorphisms (SNP) in exon 8 (+6424G>T) using quantitative TaqMan, real-time polymerase chain reaction (PCR). In addition, we measured the ficolin-2 plasma levels at two time points: on admission (t 0, acute disease) and 4 weeks after treatment (t 1, healthy phase). Comparison of ficolin-2 plasma levels shows that ficolin-2 concentration is highest during acute severe disease. In addition, we determined polymorphisms in the promoter and all coding regions of FCN2 in 40 Gabonese. Linkage disequilibrium data revealed polymorphic allelic combination patterns in the FCN2 promoter region; strong allelic combinations at -986 and -4, and -557 and -64 were found. No FCN2 promoter haplotypes were significantly distributed between mild and severe cases.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalHuman Immunology
Volume72
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Genetic Polymorphisms
Malaria
Acute Disease
Single Nucleotide Polymorphism
Gabon
Linkage Disequilibrium
Phagocytosis
Genetic Promoter Regions
Protein Binding
Haplotypes
Blood Proteins
Real-Time Polymerase Chain Reaction
Exons
ficolin
Therapeutics

Keywords

  • FCN2 gene polymorphisms
  • Ficolin-2
  • Gabon
  • Malaria
  • Real time PCR

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Faik, I., Oyedeji, S. I., Md Idris, Z., de Messias-Reason, I. J., Lell, B., Kremsner, P. G., & Kun, J. F. J. (2011). Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria. Human Immunology, 72(1), 74-79. https://doi.org/10.1016/j.humimm.2010.10.003

Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria. / Faik, Imad; Oyedeji, Segun I.; Md Idris, Zulkarnain; de Messias-Reason, Iara J.; Lell, Bertrand; Kremsner, Peter G.; Kun, Jürgen F J.

In: Human Immunology, Vol. 72, No. 1, 01.2011, p. 74-79.

Research output: Contribution to journalArticle

Faik, I, Oyedeji, SI, Md Idris, Z, de Messias-Reason, IJ, Lell, B, Kremsner, PG & Kun, JFJ 2011, 'Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria', Human Immunology, vol. 72, no. 1, pp. 74-79. https://doi.org/10.1016/j.humimm.2010.10.003
Faik I, Oyedeji SI, Md Idris Z, de Messias-Reason IJ, Lell B, Kremsner PG et al. Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria. Human Immunology. 2011 Jan;72(1):74-79. https://doi.org/10.1016/j.humimm.2010.10.003
Faik, Imad ; Oyedeji, Segun I. ; Md Idris, Zulkarnain ; de Messias-Reason, Iara J. ; Lell, Bertrand ; Kremsner, Peter G. ; Kun, Jürgen F J. / Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria. In: Human Immunology. 2011 ; Vol. 72, No. 1. pp. 74-79.
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