Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold

Munirah Sha'Ban, Sun Jung Yoon, Youn Kyung Ko, Hyun Jung Ha, Soon Hee Kim, Jung Won So, Ruszymah Idrus, Gilson Khang

Research output: Contribution to journalArticle

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Abstract

Previously, we have proven that fibrin and poly(lactic-co-glycolic acid) (PLGA) scaffolds facilitate cell proliferation, matrix production and early chondrogenesis of rabbit articular chondrocytes in in vitro and in vivo experiments. In this study, we evaluated the potential of fibrin/PLGA scaffold for intervertebral disc (IVD) tissue engineering using annulus fibrosus (AF) and nucleus pulposus (NP) cells in relation to potential clinical application. PLGA scaffolds were soaked in cells-fibrin suspension and polymerized by dropping thrombin-sodium chloride (CaCl2) solution. A PLGA-cell complex without fibrin was used as control. Higher cellular proliferation activity was observed in fibrin/PLGA-seeded AF and NP cells at each time point of 3, 7, 14 and 7 days using the MTT assay. After 3 weeks in vitro incubation, fibrin/PLGA exhibited a firmer gross morphology than PLGA groups. A significant cartilaginous tissue formation was observed in fibrin/PLGA, as proven by the development of cells cluster of various sizes and three-dimensional (3D) cartilaginous histoarchitecture and the presence of proteoglycan-rich matrix and glycosaminoglycan (GAG). The sGAG production measured by 1,9-dimethylmethylene blue (DMMB) assay revealed greater sGAG production in fibrin/PLGA than PLGA group. Immunohistochemical analyses showed expressions of collagen type II, aggrecan core protein and collagen type I genes throughout in vitro culture in both fibrin/PLGA and PLGA. In conclusion, fibrin promotes cell proliferation, stable in vitro tissue morphology, superior cartilaginous tissue formation and sGAG production of AF and NP cells cultured in PLGA scaffold. The 3D porous PLGA scaffold-cell complexes using fibrin can provide a vehicle for delivery of cells to regenerate tissue-engineered IVD tissue.

Original languageEnglish
Pages (from-to)1219-1237
Number of pages19
JournalJournal of Biomaterials Science, Polymer Edition
Volume19
Issue number9
DOIs
Publication statusPublished - 1 Sep 2008

Fingerprint

Intervertebral Disc
Fibrin
Scaffolds
Cultured Cells
Acids
Tissue
Cell Proliferation
Cell proliferation
Scaffolds (biology)
polylactic acid-polyglycolic acid copolymer
Milk
Collagen
Assays
Aggrecans
Chondrogenesis
Collagen Type II
Proteoglycans
Sodium chloride
Tissue Engineering
Chondrocytes

Keywords

  • Annulus fibrosus
  • Fibrin
  • Intervertebral disc
  • Nucleus pulposus
  • Poly(lactic-co-glycolic acid)
  • Tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials

Cite this

Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold. / Sha'Ban, Munirah; Yoon, Sun Jung; Ko, Youn Kyung; Ha, Hyun Jung; Kim, Soon Hee; So, Jung Won; Idrus, Ruszymah; Khang, Gilson.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 19, No. 9, 01.09.2008, p. 1219-1237.

Research output: Contribution to journalArticle

Sha'Ban, Munirah ; Yoon, Sun Jung ; Ko, Youn Kyung ; Ha, Hyun Jung ; Kim, Soon Hee ; So, Jung Won ; Idrus, Ruszymah ; Khang, Gilson. / Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold. In: Journal of Biomaterials Science, Polymer Edition. 2008 ; Vol. 19, No. 9. pp. 1219-1237.
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AU - Ha, Hyun Jung

AU - Kim, Soon Hee

AU - So, Jung Won

AU - Idrus, Ruszymah

AU - Khang, Gilson

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