Fermentative production of xylitol: A first trial on xylose bifurcation

Tee Zhao Kang, Siti Hajar Mohammad, Abdul Munir Abd. Murad, Rosli Md Illias, Jamaliah Md Jahim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background/Objectives: Xylitol production through chemical processes pathway involves high energy usage and production cost. Alternative method via microbial biotransformation and biocatalyst offer more sustainable and environmental friendly feedstock to be used for xylitol production. Methods: Production of xylitol by Aspergillus niger PY11 using different conditions on 2 carbon source, glucose and xylose, were done for the development of this research. Batch fermentation of A. niger PY11 was conducted for 4 days or 96 hours in temperature set at 30°C and agitation speed of 200 rpm. Samples were taken at 12 hours interval, filtered and analyzed for cell biomass, remaining sugar and D-xylitol concentration. The production of biomass and xylitol was monitored through dry-mass weight of mycelium and by HPLC, respectively. Findings: From the results of the utilization of single carbon source, fermentation of D-xylose produced the highest xylitol yield, which was 0.101 g xylitol/g D-xylose consumed, with the xylitol titre of 1.139 g/l was obtained (equivalent to 0.482 g xylitol/g biomass). However, the highest cell growth was observed when fermentation were conducted using a mixture of D-xylose and D-glucose at the ratio of 3:1, which resulted the biomass yield of 0.239 g biomass/g D-xylose (equivalent to 0.211 g xylitol/g biomass). Total amount of 44.94% of added D-xylose was consumed during the fermentation. Applications/Improvements: This paper shown that the addition of glucose had resulted higher biomass growth of A.nigerPY11, thus subsequently increased the bioconversion of xylose to xylitol.

Original languageEnglish
Article number95234
JournalIndian Journal of Science and Technology
Volume9
Issue number21
DOIs
Publication statusPublished - 2016

Fingerprint

xylitol
xylose
biomass
fermentation
biotransformation
Aspergillus niger
glucose
batch fermentation
carbon
agitation
feedstocks
production costs
mycelium
cell growth
biomass production

Keywords

  • Aspergillus niger
  • Co-substrate
  • Fermentation
  • Xylitol

ASJC Scopus subject areas

  • General

Cite this

Fermentative production of xylitol : A first trial on xylose bifurcation. / Kang, Tee Zhao; Mohammad, Siti Hajar; Abd. Murad, Abdul Munir; Illias, Rosli Md; Md Jahim, Jamaliah.

In: Indian Journal of Science and Technology, Vol. 9, No. 21, 95234, 2016.

Research output: Contribution to journalArticle

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abstract = "Background/Objectives: Xylitol production through chemical processes pathway involves high energy usage and production cost. Alternative method via microbial biotransformation and biocatalyst offer more sustainable and environmental friendly feedstock to be used for xylitol production. Methods: Production of xylitol by Aspergillus niger PY11 using different conditions on 2 carbon source, glucose and xylose, were done for the development of this research. Batch fermentation of A. niger PY11 was conducted for 4 days or 96 hours in temperature set at 30°C and agitation speed of 200 rpm. Samples were taken at 12 hours interval, filtered and analyzed for cell biomass, remaining sugar and D-xylitol concentration. The production of biomass and xylitol was monitored through dry-mass weight of mycelium and by HPLC, respectively. Findings: From the results of the utilization of single carbon source, fermentation of D-xylose produced the highest xylitol yield, which was 0.101 g xylitol/g D-xylose consumed, with the xylitol titre of 1.139 g/l was obtained (equivalent to 0.482 g xylitol/g biomass). However, the highest cell growth was observed when fermentation were conducted using a mixture of D-xylose and D-glucose at the ratio of 3:1, which resulted the biomass yield of 0.239 g biomass/g D-xylose (equivalent to 0.211 g xylitol/g biomass). Total amount of 44.94{\%} of added D-xylose was consumed during the fermentation. Applications/Improvements: This paper shown that the addition of glucose had resulted higher biomass growth of A.nigerPY11, thus subsequently increased the bioconversion of xylose to xylitol.",
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