Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Najeeb Kaid Nasser Al-Shorgani, Mohd. Sahaid Kalil, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Acetone-butanol-ethanol (ABE) was produced in Clostridium saccharoperbutylacetonicum N1-4 using sago starch as a substrate, and the effects of nutrient supplementation on ABE production were studied. Using sago starch as a substrate led to higher butanol production in C. saccharoperbutylacetonicum N1-4 when compared to other starchy materials as substrates. TYA medium supplemented with sago starch led to enhanced ABE and butanol production; however, P2 medium improved ABE production more than TYA medium. The highest ABE and butanol production was achieved with P2 medium supplemented with 70 g/L of sago starch. Using these fermentation conditions, ABE and butanol were produced at 16.65 and 9.83 g/L, respectively, and productivity was 0.15 g/L h for ABE and 0.09 g/L h for butanol. Using P2 medium supplemented with either 50 g/L enzymatically hydrolyzed sago starch or 70 g/L sago starch without hydrolysis, 9.81 and 9.83 g/L butanol were produced, respectively. These results reveal the importance of media formulation in the biobutanol fermentation of sago starch by C. saccharoperbutylacetonicum N1-4.

Original languageEnglish
Pages (from-to)1059-1070
Number of pages12
JournalAnnals of Microbiology
Volume62
Issue number3
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Butanols
Batch Cell Culture Techniques
Clostridium
Starch
Fermentation
Acetone
Ethanol
Hydrolysis

Keywords

  • Biobutanol
  • Clostridium saccharoperbutylacetonicum N1-4
  • Nutrient supplementation
  • Sago starch

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). / Al-Shorgani, Najeeb Kaid Nasser; Kalil, Mohd. Sahaid; Wan Yusoff, Wan Mohtar.

In: Annals of Microbiology, Vol. 62, No. 3, 09.2012, p. 1059-1070.

Research output: Contribution to journalArticle

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