Feasibility study on cytological sperm bundle assessment of F 1 progeny of irradiated male painted apple moth (Teia anartoides Walker; Lepidoptera

Lymantriidae) for the sterile insect technique

Wee Suk Ling, David Maxwell Suckling, Anne M. Barrington

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The advantage of inherited sterility over complete sterility in lepidopteran sterile insect technique programs results from the improvement of mating fitness of male-only releases with wild females, and the resulting large multiplier effect from viable but sterile progeny from every mating. The deleterious effects induced by irradiation are inherited by the F 1 generation, but it is very difficult to measure population introgression at that stage, and the alternative has been to await population suppression at the F 2 generation. This work, conducted in support of the successful elimination of painted apple moth (Teia anartoides) in New Zealand, aimed to determine the feasibility of a cytological assessment on the F 1 sperm bundles of this species, as a new forensic biosecurity tool providing information for decision support. The technique successfully distinguished the homogeneous nuclei clusters of eupyrene bundles of the normal fertile males from the heterogeneously stained nuclei clusters of the F 1 progeny. However, the challenge for the technique involved obtaining good specimens for cytological diagnosis. The percentage of positive staining results was correlated strongly with survival, which was <5 days. Moths that had spent 24h on a sticky base in a monitoring trap were equivalent to freshly killed specimens, but the efficacy of the technique decreased after that. Some specimens that were 'dead' to the naked eye but potentially alive internally produced reliable results. The technique may be potentially useful as a forensic biosecurity tool in the future when a communicating trap is deployed, ensuring that fresh specimens could be used to monitor the success of inherited sterility in population suppression or eradication.

Original languageEnglish
Pages (from-to)269-275
Number of pages7
JournalAustralian Journal of Entomology
Volume50
Issue number3
DOIs
Publication statusPublished - Aug 2011

Fingerprint

sterile insect technique
Lymantriidae
sterility
feasibility study
sperm
moth
Lepidoptera
spermatozoa
insect
biosecurity
traps
introgression
multipliers
monitoring
irradiation
fitness
methodology
moths
eyes
Teia anartoides

Keywords

  • Eupyrene sperm
  • Inherited sterility
  • Moth longevity
  • Painted apple moth
  • Sterile insect technique

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science

Cite this

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title = "Feasibility study on cytological sperm bundle assessment of F 1 progeny of irradiated male painted apple moth (Teia anartoides Walker; Lepidoptera: Lymantriidae) for the sterile insect technique",
abstract = "The advantage of inherited sterility over complete sterility in lepidopteran sterile insect technique programs results from the improvement of mating fitness of male-only releases with wild females, and the resulting large multiplier effect from viable but sterile progeny from every mating. The deleterious effects induced by irradiation are inherited by the F 1 generation, but it is very difficult to measure population introgression at that stage, and the alternative has been to await population suppression at the F 2 generation. This work, conducted in support of the successful elimination of painted apple moth (Teia anartoides) in New Zealand, aimed to determine the feasibility of a cytological assessment on the F 1 sperm bundles of this species, as a new forensic biosecurity tool providing information for decision support. The technique successfully distinguished the homogeneous nuclei clusters of eupyrene bundles of the normal fertile males from the heterogeneously stained nuclei clusters of the F 1 progeny. However, the challenge for the technique involved obtaining good specimens for cytological diagnosis. The percentage of positive staining results was correlated strongly with survival, which was <5 days. Moths that had spent 24h on a sticky base in a monitoring trap were equivalent to freshly killed specimens, but the efficacy of the technique decreased after that. Some specimens that were 'dead' to the naked eye but potentially alive internally produced reliable results. The technique may be potentially useful as a forensic biosecurity tool in the future when a communicating trap is deployed, ensuring that fresh specimens could be used to monitor the success of inherited sterility in population suppression or eradication.",
keywords = "Eupyrene sperm, Inherited sterility, Moth longevity, Painted apple moth, Sterile insect technique",
author = "{Suk Ling}, Wee and Suckling, {David Maxwell} and Barrington, {Anne M.}",
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N2 - The advantage of inherited sterility over complete sterility in lepidopteran sterile insect technique programs results from the improvement of mating fitness of male-only releases with wild females, and the resulting large multiplier effect from viable but sterile progeny from every mating. The deleterious effects induced by irradiation are inherited by the F 1 generation, but it is very difficult to measure population introgression at that stage, and the alternative has been to await population suppression at the F 2 generation. This work, conducted in support of the successful elimination of painted apple moth (Teia anartoides) in New Zealand, aimed to determine the feasibility of a cytological assessment on the F 1 sperm bundles of this species, as a new forensic biosecurity tool providing information for decision support. The technique successfully distinguished the homogeneous nuclei clusters of eupyrene bundles of the normal fertile males from the heterogeneously stained nuclei clusters of the F 1 progeny. However, the challenge for the technique involved obtaining good specimens for cytological diagnosis. The percentage of positive staining results was correlated strongly with survival, which was <5 days. Moths that had spent 24h on a sticky base in a monitoring trap were equivalent to freshly killed specimens, but the efficacy of the technique decreased after that. Some specimens that were 'dead' to the naked eye but potentially alive internally produced reliable results. The technique may be potentially useful as a forensic biosecurity tool in the future when a communicating trap is deployed, ensuring that fresh specimens could be used to monitor the success of inherited sterility in population suppression or eradication.

AB - The advantage of inherited sterility over complete sterility in lepidopteran sterile insect technique programs results from the improvement of mating fitness of male-only releases with wild females, and the resulting large multiplier effect from viable but sterile progeny from every mating. The deleterious effects induced by irradiation are inherited by the F 1 generation, but it is very difficult to measure population introgression at that stage, and the alternative has been to await population suppression at the F 2 generation. This work, conducted in support of the successful elimination of painted apple moth (Teia anartoides) in New Zealand, aimed to determine the feasibility of a cytological assessment on the F 1 sperm bundles of this species, as a new forensic biosecurity tool providing information for decision support. The technique successfully distinguished the homogeneous nuclei clusters of eupyrene bundles of the normal fertile males from the heterogeneously stained nuclei clusters of the F 1 progeny. However, the challenge for the technique involved obtaining good specimens for cytological diagnosis. The percentage of positive staining results was correlated strongly with survival, which was <5 days. Moths that had spent 24h on a sticky base in a monitoring trap were equivalent to freshly killed specimens, but the efficacy of the technique decreased after that. Some specimens that were 'dead' to the naked eye but potentially alive internally produced reliable results. The technique may be potentially useful as a forensic biosecurity tool in the future when a communicating trap is deployed, ensuring that fresh specimens could be used to monitor the success of inherited sterility in population suppression or eradication.

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