Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture

Yasmin Anum Mohd Yusof, Junaida Maimunah Hassan Basari, Nor Ashikeen Mukti, Razali Sabuddin, A. Razak Muda, Suhaina Sulaiman, Suzana Makpol, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Varying culture methods of Chlorella vulgaris (CV) has been associated with different nutrient composition. The aim of this study was to investigate the fatty acid contents and other nutrients of CV subjected to various culturing conditions. We found that CV cultured under 24 h light and 10% CO2 showed the best growth rates and contained higher lipid, protein and moisture contents compared to other culture conditions. Interestingly, the content of fatty acids of CV was dependent on the amount of CO2. Fatty acid analysis of CV by gas chromatography-mass spectrometry (GC-MS) showed the presence of cis-10-pentadecanoic acid (C15:1), palmitic acid (C16:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), stearic acid (C18:0), oleic acid (C18:1n9c), linoleic acid (C18:2n6c), linolenic acids (C18:3n3) and arachidic acid (C20:0). Remarkably, polyunsaturated fatty acids (linoleic and linolenic acids) are found in abundance compared to other fatty acids in CV. The concentrations of palmitic, oleic, linoleic and linolenic acids increased when the amount of carbon dioxide was raised from 1 to 10% under both culture conditions (12 and 24 h light). This study shows the possibility of modifying lipid contents in freshwater microalgae by varying the amount of carbon dioxide and light.

Original languageEnglish
Pages (from-to)13536-13542
Number of pages7
JournalAfrican Journal of Biotechnology
Volume10
Issue number62
Publication statusPublished - 12 Oct 2011

Fingerprint

Chlorella vulgaris
Microalgae
microalgae
Carbon Dioxide
Fatty Acids
fatty acid composition
carbon dioxide
linolenic acid
Linolenic Acids
linoleic acid
Light
palmitic acid
oleic acid
acids
Palmitic Acids
lipid content
Oleic Acids
Linoleic Acids
fatty acids
Lipids

Keywords

  • Carbon dioxide
  • Chlorella vulgaris
  • Fatty acids
  • Microalgae
  • Minerals
  • Vitamins

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

Cite this

Mohd Yusof, Y. A., Basari, J. M. H., Mukti, N. A., Sabuddin, R., Muda, A. R., Sulaiman, S., ... Ngah, W. Z. W. (2011). Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture. African Journal of Biotechnology, 10(62), 13536-13542.

Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture. / Mohd Yusof, Yasmin Anum; Basari, Junaida Maimunah Hassan; Mukti, Nor Ashikeen; Sabuddin, Razali; Muda, A. Razak; Sulaiman, Suhaina; Makpol, Suzana; Ngah, Wan Zurinah Wan.

In: African Journal of Biotechnology, Vol. 10, No. 62, 12.10.2011, p. 13536-13542.

Research output: Contribution to journalArticle

Mohd Yusof, YA, Basari, JMH, Mukti, NA, Sabuddin, R, Muda, AR, Sulaiman, S, Makpol, S & Ngah, WZW 2011, 'Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture', African Journal of Biotechnology, vol. 10, no. 62, pp. 13536-13542.
Mohd Yusof YA, Basari JMH, Mukti NA, Sabuddin R, Muda AR, Sulaiman S et al. Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture. African Journal of Biotechnology. 2011 Oct 12;10(62):13536-13542.
Mohd Yusof, Yasmin Anum ; Basari, Junaida Maimunah Hassan ; Mukti, Nor Ashikeen ; Sabuddin, Razali ; Muda, A. Razak ; Sulaiman, Suhaina ; Makpol, Suzana ; Ngah, Wan Zurinah Wan. / Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture. In: African Journal of Biotechnology. 2011 ; Vol. 10, No. 62. pp. 13536-13542.
@article{33989296dab4484187f0547ce98fda5c,
title = "Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture",
abstract = "Varying culture methods of Chlorella vulgaris (CV) has been associated with different nutrient composition. The aim of this study was to investigate the fatty acid contents and other nutrients of CV subjected to various culturing conditions. We found that CV cultured under 24 h light and 10{\%} CO2 showed the best growth rates and contained higher lipid, protein and moisture contents compared to other culture conditions. Interestingly, the content of fatty acids of CV was dependent on the amount of CO2. Fatty acid analysis of CV by gas chromatography-mass spectrometry (GC-MS) showed the presence of cis-10-pentadecanoic acid (C15:1), palmitic acid (C16:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), stearic acid (C18:0), oleic acid (C18:1n9c), linoleic acid (C18:2n6c), linolenic acids (C18:3n3) and arachidic acid (C20:0). Remarkably, polyunsaturated fatty acids (linoleic and linolenic acids) are found in abundance compared to other fatty acids in CV. The concentrations of palmitic, oleic, linoleic and linolenic acids increased when the amount of carbon dioxide was raised from 1 to 10{\%} under both culture conditions (12 and 24 h light). This study shows the possibility of modifying lipid contents in freshwater microalgae by varying the amount of carbon dioxide and light.",
keywords = "Carbon dioxide, Chlorella vulgaris, Fatty acids, Microalgae, Minerals, Vitamins",
author = "{Mohd Yusof}, {Yasmin Anum} and Basari, {Junaida Maimunah Hassan} and Mukti, {Nor Ashikeen} and Razali Sabuddin and Muda, {A. Razak} and Suhaina Sulaiman and Suzana Makpol and Ngah, {Wan Zurinah Wan}",
year = "2011",
month = "10",
day = "12",
language = "English",
volume = "10",
pages = "13536--13542",
journal = "African Journal of Biotechnology",
issn = "1684-5315",
publisher = "Academic Journals",
number = "62",

}

TY - JOUR

T1 - Fatty acids composition of microalgae chlorella vulgaris can be modulated by varying carbon dioxide concentration in outdoor culture

AU - Mohd Yusof, Yasmin Anum

AU - Basari, Junaida Maimunah Hassan

AU - Mukti, Nor Ashikeen

AU - Sabuddin, Razali

AU - Muda, A. Razak

AU - Sulaiman, Suhaina

AU - Makpol, Suzana

AU - Ngah, Wan Zurinah Wan

PY - 2011/10/12

Y1 - 2011/10/12

N2 - Varying culture methods of Chlorella vulgaris (CV) has been associated with different nutrient composition. The aim of this study was to investigate the fatty acid contents and other nutrients of CV subjected to various culturing conditions. We found that CV cultured under 24 h light and 10% CO2 showed the best growth rates and contained higher lipid, protein and moisture contents compared to other culture conditions. Interestingly, the content of fatty acids of CV was dependent on the amount of CO2. Fatty acid analysis of CV by gas chromatography-mass spectrometry (GC-MS) showed the presence of cis-10-pentadecanoic acid (C15:1), palmitic acid (C16:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), stearic acid (C18:0), oleic acid (C18:1n9c), linoleic acid (C18:2n6c), linolenic acids (C18:3n3) and arachidic acid (C20:0). Remarkably, polyunsaturated fatty acids (linoleic and linolenic acids) are found in abundance compared to other fatty acids in CV. The concentrations of palmitic, oleic, linoleic and linolenic acids increased when the amount of carbon dioxide was raised from 1 to 10% under both culture conditions (12 and 24 h light). This study shows the possibility of modifying lipid contents in freshwater microalgae by varying the amount of carbon dioxide and light.

AB - Varying culture methods of Chlorella vulgaris (CV) has been associated with different nutrient composition. The aim of this study was to investigate the fatty acid contents and other nutrients of CV subjected to various culturing conditions. We found that CV cultured under 24 h light and 10% CO2 showed the best growth rates and contained higher lipid, protein and moisture contents compared to other culture conditions. Interestingly, the content of fatty acids of CV was dependent on the amount of CO2. Fatty acid analysis of CV by gas chromatography-mass spectrometry (GC-MS) showed the presence of cis-10-pentadecanoic acid (C15:1), palmitic acid (C16:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), stearic acid (C18:0), oleic acid (C18:1n9c), linoleic acid (C18:2n6c), linolenic acids (C18:3n3) and arachidic acid (C20:0). Remarkably, polyunsaturated fatty acids (linoleic and linolenic acids) are found in abundance compared to other fatty acids in CV. The concentrations of palmitic, oleic, linoleic and linolenic acids increased when the amount of carbon dioxide was raised from 1 to 10% under both culture conditions (12 and 24 h light). This study shows the possibility of modifying lipid contents in freshwater microalgae by varying the amount of carbon dioxide and light.

KW - Carbon dioxide

KW - Chlorella vulgaris

KW - Fatty acids

KW - Microalgae

KW - Minerals

KW - Vitamins

UR - http://www.scopus.com/inward/record.url?scp=80054763175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054763175&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:80054763175

VL - 10

SP - 13536

EP - 13542

JO - African Journal of Biotechnology

JF - African Journal of Biotechnology

SN - 1684-5315

IS - 62

ER -