Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda)

Chew Hee Ng, Han Kiat Alan Ong, Chiak Wu Kong, Kong Wai Tan, Raja Noor Zaliha Raja Abd Rahman, Bohari Mohd. Yamin, Seik Weng Ng

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV-visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N′-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na2H2EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change.

Original languageEnglish
Pages (from-to)3118-3126
Number of pages9
JournalPolyhedron
Volume25
Issue number16
DOIs
Publication statusPublished - 13 Nov 2006

Fingerprint

DNA Cleavage
Coordination Complexes
Metal complexes
cleavage
Buffers
DNA
deoxyribonucleic acid
Crystal structure
crystal structure
buffers
Cobalt
metals
Hydrogen Peroxide
Spectrum Analysis
Plasmids
plasmids
hydrogen peroxide
Hydrogen peroxide
Conformations
Assays

Keywords

  • (N,N′-ethylenediaminediacetato)metal(II)
  • Crystal structure
  • DNA cleavage
  • Hydrogen peroxide
  • Nucleolytic efficiency

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

Ng, C. H., Alan Ong, H. K., Kong, C. W., Tan, K. W., Rahman, R. N. Z. R. A., Mohd. Yamin, B., & Ng, S. W. (2006). Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda). Polyhedron, 25(16), 3118-3126. https://doi.org/10.1016/j.poly.2006.05.020

Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda). / Ng, Chew Hee; Alan Ong, Han Kiat; Kong, Chiak Wu; Tan, Kong Wai; Rahman, Raja Noor Zaliha Raja Abd; Mohd. Yamin, Bohari; Ng, Seik Weng.

In: Polyhedron, Vol. 25, No. 16, 13.11.2006, p. 3118-3126.

Research output: Contribution to journalArticle

Ng, Chew Hee ; Alan Ong, Han Kiat ; Kong, Chiak Wu ; Tan, Kong Wai ; Rahman, Raja Noor Zaliha Raja Abd ; Mohd. Yamin, Bohari ; Ng, Seik Weng. / Factors affecting the nucleolytic cleavage of DNA by (N,N′-ethylenendiaminediacetato)metal(II) complexes, M(edda). Crystal structure of Co(edda). In: Polyhedron. 2006 ; Vol. 25, No. 16. pp. 3118-3126.
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