Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)

Hoi Ling Seng, Han Kiat Alan Ong, Raja Noor Zaliha Raja Abd Rahman, Bohari Mohd. Yamin, Edward R T Tiekink, Kong Wai Tan, Mohd Jamil Maah, Ignez Caracelli, Chew Hee Ng

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)6, ds(AT)6 and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is intercalation and there is selectivity towards AT-sequence and stacking preference for A/A parallel or diagonal adjacent base steps in their intercalation. The nucleolytic properties of these complexes were investigated and the factors affecting the extent of cleavage were determined to be: concentration of complex, the nature of metal(II) ion, type of buffer, pH of buffer, incubation time, incubation temperature, and the presence of hydrogen peroxide or ascorbic acid as exogenous reagents. The fluorescence property of these complexes and its origin were also investigated. The crystal structure of the Zn(phen)(edda) complex is reported in which the zinc atom displays a distorted trans-N4O2 octahedral geometry; the crystal packing features double layers of complex molecules held together by extensive hydrogen bonding that inter-digitate with adjacent double layers via π...π interactions between 1,10-phenanthroline residues. The structure is compared with that of the recently described copper(II) analogue and, with the latter, included in molecular modeling.

Original languageEnglish
Pages (from-to)1997-2011
Number of pages15
JournalJournal of Inorganic Biochemistry
Volume102
Issue number11
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Molecular modeling
Coordination Complexes
Intercalation
Molecular Structure
Molecular structure
Buffers
Crystal structure
DNA
Hydrogen Bonding
Oligonucleotides
Hydrogen Peroxide
Ascorbic Acid
Metal ions
Zinc
Copper
Spectrum Analysis
Hydrogen bonds
Fluorescence
Spectroscopy
Ions

Keywords

  • DNA binding
  • Intercalation
  • Metal(II) ternary complexes
  • Molecular modeling
  • Nucleolytic

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda). / Seng, Hoi Ling; Ong, Han Kiat Alan; Rahman, Raja Noor Zaliha Raja Abd; Mohd. Yamin, Bohari; Tiekink, Edward R T; Tan, Kong Wai; Maah, Mohd Jamil; Caracelli, Ignez; Ng, Chew Hee.

In: Journal of Inorganic Biochemistry, Vol. 102, No. 11, 11.2008, p. 1997-2011.

Research output: Contribution to journalArticle

Seng, Hoi Ling ; Ong, Han Kiat Alan ; Rahman, Raja Noor Zaliha Raja Abd ; Mohd. Yamin, Bohari ; Tiekink, Edward R T ; Tan, Kong Wai ; Maah, Mohd Jamil ; Caracelli, Ignez ; Ng, Chew Hee. / Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda). In: Journal of Inorganic Biochemistry. 2008 ; Vol. 102, No. 11. pp. 1997-2011.
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