Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production

Anis Surayani Mat Yaacob, Nurul Aishah Mazlan, Kamaliah Abdul Samad, Syed Mohd Saufi, Hafizuddin Wan Yussof, Jamaliah Md Jahim

Research output: Contribution to journalArticle

Abstract

Enzymatic hydrolysis has become outstanding technology in converting lignocellulosic biomass to its xylose monomer for xylooligosaccharides (XOS) production. The present work involves an investigation on the effects of enzyme loading, agitation speed, substrate loading, temperature and hydrolysis time on enzymatic hydrolysis for XOS production. Pretreated oil palm frond bagasse by dilute nitric acid was used for enzymatic hydrolysis using Cellic HTec2. The effects of factors were analyzed by half fractional factorial design 25-1 using Design Expert with Response Surface Methodology (RSM) to achieve maximum XOS production. The results revealed that the best enzymatic hydrolysis condition yielded 4.13 mg/L of XOS when conducted at 5% (w/v) of substrate loading, 50 U/mL enzyme loading with 200 rpm agitation speed and 55°C for 4 hours of hydrolysis time. Two factors that contributed to the highest production of XOS were substrate loading and enzyme loading. The model obtained in this present research is significant with p-value < 0.0001 and R-squared of 0.9545. It is recommended that model had a maximum point which is possible for the optimization process later. Overall, the findings of this study suggest that Cellic HTec2 is a suitable candidate for enzymatic hydrolysis of pretreated OPFB for higher XOS production.

Original languageEnglish
Pages (from-to)122-129
Number of pages8
JournalAsian Journal of Agriculture and Biology
Volume7
Issue number1
Publication statusPublished - 1 Jan 2019

Fingerprint

xylooligosaccharides
bagasse
Palm oil
Enzymatic hydrolysis
Elaeis guineensis
fronds
enzymatic hydrolysis
Oils
Hydrolysis
agitation
Substrates
Enzymes
enzymes
hydrolysis
Nitric Acid
nitric acid
Xylose
response surface methodology
xylose
xylooligosaccharide

Keywords

  • Cellic HTec2
  • Enzymatic hydrolysis
  • Oil palm frond bagasse
  • Response surface methodology
  • Xylooligosaccharides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production. / Mat Yaacob, Anis Surayani; Mazlan, Nurul Aishah; Samad, Kamaliah Abdul; Saufi, Syed Mohd; Wan Yussof, Hafizuddin; Md Jahim, Jamaliah.

In: Asian Journal of Agriculture and Biology, Vol. 7, No. 1, 01.01.2019, p. 122-129.

Research output: Contribution to journalArticle

Mat Yaacob, Anis Surayani ; Mazlan, Nurul Aishah ; Samad, Kamaliah Abdul ; Saufi, Syed Mohd ; Wan Yussof, Hafizuddin ; Md Jahim, Jamaliah. / Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production. In: Asian Journal of Agriculture and Biology. 2019 ; Vol. 7, No. 1. pp. 122-129.
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