Factors affecting enzymatic hydrolysis from pretreated fibre pressed oil palm frond using sacchariseb C6

Fatin Syazwana Hashim, Wan Mohd Hafizuddin Wan Yussof, Mah Kah Hong, Nurul Fatihah Mohamad Roli, Syed Mohd Saufi Tuan Chik, Mazrul Nizam Abu Seman, Abdul Wahab Mohammad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, fibre pressed oil palm frond (FPOPF) was introduced as the raw material for the production of glucose using Sacchariseb C6 to maximise the utilisation of oil palm waste. Sacchariseb C6 is a commercial cellulase blended enzyme from Advanced Enzymes Technology. In order to achieve maximum glucose production, a factorial analysis 25-1 using response surface methodology (RSM) was employed to screen the best enzymatic hydrolysis condition by varying the parameters such as agitation speed, enzyme loading, glucan loading, temperature and hydrolysis time. FPOPF was treated with 4.42% (w/v) of sodium hydroxide at 100°C prior to the enzymatic hydrolysis. Raw FPOPF consists of 40.7% glucan, 26.1% xylan, 26.2% lignin, 1.8% ash and 4.5% extractives. On the other hand, pretreated FPOPF consists of 61.4% glucan, 20.4% xylan, 13.3% lignin, 1.3% ash and 0.3% extractives. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4% (w/w) of glucan loading, temperature at 55°C and 72 h of reaction time. The model obtained from RSM was significant with p-value < 0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimisation process.

Original languageEnglish
Pages (from-to)281-295
Number of pages15
JournalJournal of Physical Science
Volume28
DOIs
Publication statusPublished - 2017

Fingerprint

Palm oil
Enzymatic hydrolysis
Glucans
hydrolysis
Ashes
oils
enzymes
Enzymes
fibers
glucose
Fibers
Glucose
Xylans
agitation
lignin
Lignin
ashes
methodology
Sodium Hydroxide
Cellulase

Keywords

  • Enzymatic hydrolysis
  • Factorial analysis
  • Fibre pressed oil palm frond
  • Response surface methodology
  • Sacchariseb C6

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Factors affecting enzymatic hydrolysis from pretreated fibre pressed oil palm frond using sacchariseb C6. / Hashim, Fatin Syazwana; Yussof, Wan Mohd Hafizuddin Wan; Hong, Mah Kah; Roli, Nurul Fatihah Mohamad; Chik, Syed Mohd Saufi Tuan; Seman, Mazrul Nizam Abu; Mohammad, Abdul Wahab.

In: Journal of Physical Science, Vol. 28, 2017, p. 281-295.

Research output: Contribution to journalArticle

Hashim, Fatin Syazwana ; Yussof, Wan Mohd Hafizuddin Wan ; Hong, Mah Kah ; Roli, Nurul Fatihah Mohamad ; Chik, Syed Mohd Saufi Tuan ; Seman, Mazrul Nizam Abu ; Mohammad, Abdul Wahab. / Factors affecting enzymatic hydrolysis from pretreated fibre pressed oil palm frond using sacchariseb C6. In: Journal of Physical Science. 2017 ; Vol. 28. pp. 281-295.
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abstract = "In this study, fibre pressed oil palm frond (FPOPF) was introduced as the raw material for the production of glucose using Sacchariseb C6 to maximise the utilisation of oil palm waste. Sacchariseb C6 is a commercial cellulase blended enzyme from Advanced Enzymes Technology. In order to achieve maximum glucose production, a factorial analysis 25-1 using response surface methodology (RSM) was employed to screen the best enzymatic hydrolysis condition by varying the parameters such as agitation speed, enzyme loading, glucan loading, temperature and hydrolysis time. FPOPF was treated with 4.42{\%} (w/v) of sodium hydroxide at 100°C prior to the enzymatic hydrolysis. Raw FPOPF consists of 40.7{\%} glucan, 26.1{\%} xylan, 26.2{\%} lignin, 1.8{\%} ash and 4.5{\%} extractives. On the other hand, pretreated FPOPF consists of 61.4{\%} glucan, 20.4{\%} xylan, 13.3{\%} lignin, 1.3{\%} ash and 0.3{\%} extractives. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4{\%} (w/w) of glucan loading, temperature at 55°C and 72 h of reaction time. The model obtained from RSM was significant with p-value < 0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimisation process.",
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AU - Yussof, Wan Mohd Hafizuddin Wan

AU - Hong, Mah Kah

AU - Roli, Nurul Fatihah Mohamad

AU - Chik, Syed Mohd Saufi Tuan

AU - Seman, Mazrul Nizam Abu

AU - Mohammad, Abdul Wahab

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AB - In this study, fibre pressed oil palm frond (FPOPF) was introduced as the raw material for the production of glucose using Sacchariseb C6 to maximise the utilisation of oil palm waste. Sacchariseb C6 is a commercial cellulase blended enzyme from Advanced Enzymes Technology. In order to achieve maximum glucose production, a factorial analysis 25-1 using response surface methodology (RSM) was employed to screen the best enzymatic hydrolysis condition by varying the parameters such as agitation speed, enzyme loading, glucan loading, temperature and hydrolysis time. FPOPF was treated with 4.42% (w/v) of sodium hydroxide at 100°C prior to the enzymatic hydrolysis. Raw FPOPF consists of 40.7% glucan, 26.1% xylan, 26.2% lignin, 1.8% ash and 4.5% extractives. On the other hand, pretreated FPOPF consists of 61.4% glucan, 20.4% xylan, 13.3% lignin, 1.3% ash and 0.3% extractives. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4% (w/w) of glucan loading, temperature at 55°C and 72 h of reaction time. The model obtained from RSM was significant with p-value < 0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimisation process.

KW - Enzymatic hydrolysis

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KW - Response surface methodology

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