Factors affecting bioactivity of secondary metabolites produced by Streptomyces sp. PT1 using Plackett-Burman design

Zidan Abduldiem Bashir, Asmat Ahmad, Shukor Md. Nor, Gires Usup

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The study aimed at evaluating the effects of certain nutrients and influence of environmental factors on growth and production of bioactive metabolite by Streptomyces PT1 isolate in submerged culture using the Plackett-Burman design. Factors considered in the study included the effect of carbon source, nitrogen source, MgSO4, FeSO4, MnCl2, ZnSO4, NaCl, pH, temperature, agitation and incubation time. Glucose and yeast extract were found to be the most suitable carbon and nitrogen sources respectively, for optimum production of growth and bioactive metabolite by the isolate. One-way analysis of variance (ANOVA) of the responses showed that FeSO4 (p=0.0215), MnCl2 (p=0.0001) and NaCl (p=0.0215) were significant factors affecting the antimicrobial activity of secondary metabolite against Bacillus subtilis (BS). Activity of secondary metabolite produced by the tested Streptomyce PT1 isolate against Vibrio parahaemoliticus (VI) was significantly affected by FeSO4 (p=0.0044) and NaCl (p=0.0044) concentration. The models established from the ANOVA analysis all presented significant values of Pmodel>F less than 0.005.

Original languageEnglish
Pages (from-to)3043-3051
Number of pages9
JournalAdvances in Environmental Biology
Volume6
Issue number12
Publication statusPublished - Nov 2012

Fingerprint

bioactivity
Streptomyces
secondary metabolite
variance analysis
secondary metabolites
Analysis of Variance
analysis of variance
metabolites
carbon
metabolite
agitation
Vibrio
nitrogen
Nitrogen
Carbon
yeast extract
Bacillus subtilis
antimicrobial activity
anti-infective agents
glucose

Keywords

  • Bioactivity
  • Physic-chemical factors
  • Plackett- Burman design
  • Secondary metabolite
  • Streptomyces sp. PT1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

Factors affecting bioactivity of secondary metabolites produced by Streptomyces sp. PT1 using Plackett-Burman design. / Bashir, Zidan Abduldiem; Ahmad, Asmat; Md. Nor, Shukor; Usup, Gires.

In: Advances in Environmental Biology, Vol. 6, No. 12, 11.2012, p. 3043-3051.

Research output: Contribution to journalArticle

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