Fabrikasi biosensor DNA porsin berasaskan kompleks rutenium bipiridina

Translated title of the contribution: Fabrication of porcine DNA biosensor based on ruthenium bipyridine complex

Nurul Izni Abdullah Halid, Emma Izzati Zakariah, Yook Heng Lee, Huda Abd Karim Nurul Huda Abd Karim, Haslina Ahmad, Siti Aishah Hasbullah

Research output: Contribution to journalArticle

Abstract

Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles (AuNPs) as transducer immobilized with poly(n-butylacrylate-N-acryloxysuccinimide) microsphere and porcine DNA probe sequences was attached onto it via the covalent bond. The ruthenium(II) complex, [Ru(bpy)2PIP]2+ PIP = 2-phenylimidazo[4,5-f[1,10-phenanthroline] intercalator has been been used to determine prcine DNA. The biosensor was measured and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The interaction of [Ru(bpy)2PIP]2+ with various DNA with different sequences, i) single stranded probe DNA, ii) after hybridization with its complementary DNA and iii) after hybridization with mismatch complementary DNA were studied. The results indicated that the interaction of [Ru(bpy)2PIP]2+ with hybridized complementary DNA gave the highest response. Thus, development of porcine DNA biosensor and influences of many factors such as [Ru(bpy)2PIP]2+ complex and DNA probe concentration, DNA probe immobilization and hybridization time, pH, ionic strength, buffer concentration and temperatures were also studied to evaluate the performance of biosensor. The concentration of [Ru(bpy)2PIP]2+ complex and DNA probe were found to be optimal at 50 µM and 2 µM, respectively. The optimal time for DNA probe immobilization and hybridization were 7 hours and 60 minutes, accordingly. The optimal condition of pH, ionic strength, buffer concentration and temperatures were at pH 7.0, 1.0 M NaCl, 0.05 M of Na-phosphate buffer and 25 °C, respectively. The linear range of different concentration complementary DNA was within the range between 1.0 × 10-13 M to 1.0 × 10-8 M. This study was first reported the used of [Ru(bpy)2PIP]2+ for detection of porcine oligonucleotides.

Original languageMalay
Pages (from-to)1020-1032
Number of pages13
JournalMalaysian Journal of Analytical Sciences
Volume20
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Ruthenium
DNA Probes
Biosensors
Fabrication
DNA
Complementary DNA
Buffers
Ionic strength
Oligonucleotides
Intercalating Agents
Covalent bonds
Voltammetry
Microspheres
Cyclic voltammetry
Labels
Transducers
Carbon
Phosphates
Temperature
Electrodes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Fabrikasi biosensor DNA porsin berasaskan kompleks rutenium bipiridina. / Halid, Nurul Izni Abdullah; Zakariah, Emma Izzati; Lee, Yook Heng; Nurul Huda Abd Karim, Huda Abd Karim; Ahmad, Haslina; Hasbullah, Siti Aishah.

In: Malaysian Journal of Analytical Sciences, Vol. 20, No. 5, 2016, p. 1020-1032.

Research output: Contribution to journalArticle

Halid, Nurul Izni Abdullah ; Zakariah, Emma Izzati ; Lee, Yook Heng ; Nurul Huda Abd Karim, Huda Abd Karim ; Ahmad, Haslina ; Hasbullah, Siti Aishah. / Fabrikasi biosensor DNA porsin berasaskan kompleks rutenium bipiridina. In: Malaysian Journal of Analytical Sciences. 2016 ; Vol. 20, No. 5. pp. 1020-1032.
@article{b175fd7390324a219ca193d6183ffec2,
title = "Fabrikasi biosensor DNA porsin berasaskan kompleks rutenium bipiridina",
abstract = "Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles (AuNPs) as transducer immobilized with poly(n-butylacrylate-N-acryloxysuccinimide) microsphere and porcine DNA probe sequences was attached onto it via the covalent bond. The ruthenium(II) complex, [Ru(bpy)2PIP]2+ PIP = 2-phenylimidazo[4,5-f[1,10-phenanthroline] intercalator has been been used to determine prcine DNA. The biosensor was measured and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The interaction of [Ru(bpy)2PIP]2+ with various DNA with different sequences, i) single stranded probe DNA, ii) after hybridization with its complementary DNA and iii) after hybridization with mismatch complementary DNA were studied. The results indicated that the interaction of [Ru(bpy)2PIP]2+ with hybridized complementary DNA gave the highest response. Thus, development of porcine DNA biosensor and influences of many factors such as [Ru(bpy)2PIP]2+ complex and DNA probe concentration, DNA probe immobilization and hybridization time, pH, ionic strength, buffer concentration and temperatures were also studied to evaluate the performance of biosensor. The concentration of [Ru(bpy)2PIP]2+ complex and DNA probe were found to be optimal at 50 µM and 2 µM, respectively. The optimal time for DNA probe immobilization and hybridization were 7 hours and 60 minutes, accordingly. The optimal condition of pH, ionic strength, buffer concentration and temperatures were at pH 7.0, 1.0 M NaCl, 0.05 M of Na-phosphate buffer and 25 °C, respectively. The linear range of different concentration complementary DNA was within the range between 1.0 × 10-13 M to 1.0 × 10-8 M. This study was first reported the used of [Ru(bpy)2PIP]2+ for detection of porcine oligonucleotides.",
keywords = "Biosensor, Porcine DNA, Ruthenium bypyridine complex",
author = "Halid, {Nurul Izni Abdullah} and Zakariah, {Emma Izzati} and Lee, {Yook Heng} and {Nurul Huda Abd Karim}, {Huda Abd Karim} and Haslina Ahmad and Hasbullah, {Siti Aishah}",
year = "2016",
doi = "10.17576/mjas-2016-2005-06",
language = "Malay",
volume = "20",
pages = "1020--1032",
journal = "Malaysian Journal of Analytical Sciences",
issn = "1394-2506",
publisher = "Faculty of Science and Technology, Universiti Kebangsaan Malaysia",
number = "5",

}

TY - JOUR

T1 - Fabrikasi biosensor DNA porsin berasaskan kompleks rutenium bipiridina

AU - Halid, Nurul Izni Abdullah

AU - Zakariah, Emma Izzati

AU - Lee, Yook Heng

AU - Nurul Huda Abd Karim, Huda Abd Karim

AU - Ahmad, Haslina

AU - Hasbullah, Siti Aishah

PY - 2016

Y1 - 2016

N2 - Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles (AuNPs) as transducer immobilized with poly(n-butylacrylate-N-acryloxysuccinimide) microsphere and porcine DNA probe sequences was attached onto it via the covalent bond. The ruthenium(II) complex, [Ru(bpy)2PIP]2+ PIP = 2-phenylimidazo[4,5-f[1,10-phenanthroline] intercalator has been been used to determine prcine DNA. The biosensor was measured and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The interaction of [Ru(bpy)2PIP]2+ with various DNA with different sequences, i) single stranded probe DNA, ii) after hybridization with its complementary DNA and iii) after hybridization with mismatch complementary DNA were studied. The results indicated that the interaction of [Ru(bpy)2PIP]2+ with hybridized complementary DNA gave the highest response. Thus, development of porcine DNA biosensor and influences of many factors such as [Ru(bpy)2PIP]2+ complex and DNA probe concentration, DNA probe immobilization and hybridization time, pH, ionic strength, buffer concentration and temperatures were also studied to evaluate the performance of biosensor. The concentration of [Ru(bpy)2PIP]2+ complex and DNA probe were found to be optimal at 50 µM and 2 µM, respectively. The optimal time for DNA probe immobilization and hybridization were 7 hours and 60 minutes, accordingly. The optimal condition of pH, ionic strength, buffer concentration and temperatures were at pH 7.0, 1.0 M NaCl, 0.05 M of Na-phosphate buffer and 25 °C, respectively. The linear range of different concentration complementary DNA was within the range between 1.0 × 10-13 M to 1.0 × 10-8 M. This study was first reported the used of [Ru(bpy)2PIP]2+ for detection of porcine oligonucleotides.

AB - Electrochemical DNA biosensor for detection of porcine oligonucleotides based on ruthenium (II) as label redox complex has been developed. The system of biosensor is based on the modified screen printed carbon electrode (SPE) with goldnanoparticles (AuNPs) as transducer immobilized with poly(n-butylacrylate-N-acryloxysuccinimide) microsphere and porcine DNA probe sequences was attached onto it via the covalent bond. The ruthenium(II) complex, [Ru(bpy)2PIP]2+ PIP = 2-phenylimidazo[4,5-f[1,10-phenanthroline] intercalator has been been used to determine prcine DNA. The biosensor was measured and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The interaction of [Ru(bpy)2PIP]2+ with various DNA with different sequences, i) single stranded probe DNA, ii) after hybridization with its complementary DNA and iii) after hybridization with mismatch complementary DNA were studied. The results indicated that the interaction of [Ru(bpy)2PIP]2+ with hybridized complementary DNA gave the highest response. Thus, development of porcine DNA biosensor and influences of many factors such as [Ru(bpy)2PIP]2+ complex and DNA probe concentration, DNA probe immobilization and hybridization time, pH, ionic strength, buffer concentration and temperatures were also studied to evaluate the performance of biosensor. The concentration of [Ru(bpy)2PIP]2+ complex and DNA probe were found to be optimal at 50 µM and 2 µM, respectively. The optimal time for DNA probe immobilization and hybridization were 7 hours and 60 minutes, accordingly. The optimal condition of pH, ionic strength, buffer concentration and temperatures were at pH 7.0, 1.0 M NaCl, 0.05 M of Na-phosphate buffer and 25 °C, respectively. The linear range of different concentration complementary DNA was within the range between 1.0 × 10-13 M to 1.0 × 10-8 M. This study was first reported the used of [Ru(bpy)2PIP]2+ for detection of porcine oligonucleotides.

KW - Biosensor

KW - Porcine DNA

KW - Ruthenium bypyridine complex

UR - http://www.scopus.com/inward/record.url?scp=84992751939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992751939&partnerID=8YFLogxK

U2 - 10.17576/mjas-2016-2005-06

DO - 10.17576/mjas-2016-2005-06

M3 - Article

VL - 20

SP - 1020

EP - 1032

JO - Malaysian Journal of Analytical Sciences

JF - Malaysian Journal of Analytical Sciences

SN - 1394-2506

IS - 5

ER -