Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds

Jaafar Abdullah, Musa Ahmad, Yook Heng Lee, Nadarajah Karuppiah, Hamidah Sidek

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fabrication of biosensor for phenolic compounds determination employing optical method is described. The biosensor involved bi-layer which consist of immobilized 3-methyl-2-benzothiazolinone hydrazone (MBTH) in composite nafion/sol-gel silicate film and immobilized tyrosinase in chitosan film. The enzymatic oxidation product of phenolic compounds were stabilized through the formation of adduct with MBTH which can be monitored spectrophotometrically at wavelength of 490 nm. The fabricated biosensor gave a linear response towards phenol, catechol and m-cresol in the concentration range of 5.0-74.0 μK 5.0-91.0 μM, 9.0-120.0 μM with detection limit of 1.9, 2.1, 4.0 μM, respectively. The biosensor was stable for a duration of at least 3 months.

Original languageEnglish
Title of host publication2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings
Pages103-106
Number of pages4
Volume2005
DOIs
Publication statusPublished - 2005
Event2005 Asian Conference on Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research - Kuala Lumpur
Duration: 5 Sep 20057 Sep 2005

Other

Other2005 Asian Conference on Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research
CityKuala Lumpur
Period5/9/057/9/05

Fingerprint

Biosensors
Fabrication
Chitosan
Phenols
Silicates
Sol-gels
Wavelength
Oxidation
Composite materials

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Abdullah, J., Ahmad, M., Lee, Y. H., Karuppiah, N., & Sidek, H. (2005). Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds. In 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings (Vol. 2005, pp. 103-106). [1564516] https://doi.org/10.1109/ASENSE.2005.1564516

Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds. / Abdullah, Jaafar; Ahmad, Musa; Lee, Yook Heng; Karuppiah, Nadarajah; Sidek, Hamidah.

2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. Vol. 2005 2005. p. 103-106 1564516.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abdullah, J, Ahmad, M, Lee, YH, Karuppiah, N & Sidek, H 2005, Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds. in 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. vol. 2005, 1564516, pp. 103-106, 2005 Asian Conference on Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research, Kuala Lumpur, 5/9/05. https://doi.org/10.1109/ASENSE.2005.1564516
Abdullah J, Ahmad M, Lee YH, Karuppiah N, Sidek H. Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds. In 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. Vol. 2005. 2005. p. 103-106. 1564516 https://doi.org/10.1109/ASENSE.2005.1564516
Abdullah, Jaafar ; Ahmad, Musa ; Lee, Yook Heng ; Karuppiah, Nadarajah ; Sidek, Hamidah. / Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds. 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. Vol. 2005 2005. pp. 103-106
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