Fabrication of an integrated microfluidic perfusion system for mixing different solutions

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Abstract

We present a simple and low cost fabrication of Microfluidic mixer using in different applications in Micro Total Analysis Systems especially forautomated perfusion of multiple solutions to a single cell culture chamber. This device consists of 6 inlets and 2 outlets with additional 3inlet/outlet channels for flow rate alteration and other mixing purposes such as electrophoresis activation, which is capable of producing mixturesof the various solutions. The volume of the microchamber is 600 pm3. All the inlets are connected to Polytetrafluoroethylene (PTFE) and siliconetubings using special Polydimethylsiloxane (PDMS) fittings technique for fluid leakage avoidance. The inputs are controlled by a programmablemicro-syringe pump in order to activate each input in any sequence of time. Typical microscopic glass slides are utilized as a substrate forfabrication of microchannels and chamber which has the minimum effect on most of biology samples. The same un-etched glass is used as coverglass for bonding purposes. Using photoresist as an etch mask instead of some deposition methods makes the procedure more convenient and costeffective compared to other expensive techniques such as Deep reactive-ion etching (DRIE), laser, etc. The baking time of photoresist is a criticalfactor for longer resistance against etchant solution which is optimized by this method. In addition the etchant concentration in wet etching processis discussed to achieve a decent surface and wall characteristics for bio-applications. A smooth channel surface with acceptable sharp wall edgesmakes this procedure suitable for many applications which vertical walls are not crucial. Different dye samples are tested inside the chamber andpromising results attained. The mixing validation is performed using image processing technique.

Original languageEnglish
Pages (from-to)30-33
Number of pages4
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume3
Issue numberSUPPL.1
Publication statusPublished - Feb 2011

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Microfluidics
Perfusion
Glass
Syringes
Polytetrafluoroethylene
Masks
Electrophoresis
Lasers
Coloring Agents
Cell Culture Techniques
Ions
Costs and Cost Analysis
Equipment and Supplies

Keywords

  • Glass
  • Micromixer
  • Photoresist
  • Wet etching

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

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abstract = "We present a simple and low cost fabrication of Microfluidic mixer using in different applications in Micro Total Analysis Systems especially forautomated perfusion of multiple solutions to a single cell culture chamber. This device consists of 6 inlets and 2 outlets with additional 3inlet/outlet channels for flow rate alteration and other mixing purposes such as electrophoresis activation, which is capable of producing mixturesof the various solutions. The volume of the microchamber is 600 pm3. All the inlets are connected to Polytetrafluoroethylene (PTFE) and siliconetubings using special Polydimethylsiloxane (PDMS) fittings technique for fluid leakage avoidance. The inputs are controlled by a programmablemicro-syringe pump in order to activate each input in any sequence of time. Typical microscopic glass slides are utilized as a substrate forfabrication of microchannels and chamber which has the minimum effect on most of biology samples. The same un-etched glass is used as coverglass for bonding purposes. Using photoresist as an etch mask instead of some deposition methods makes the procedure more convenient and costeffective compared to other expensive techniques such as Deep reactive-ion etching (DRIE), laser, etc. The baking time of photoresist is a criticalfactor for longer resistance against etchant solution which is optimized by this method. In addition the etchant concentration in wet etching processis discussed to achieve a decent surface and wall characteristics for bio-applications. A smooth channel surface with acceptable sharp wall edgesmakes this procedure suitable for many applications which vertical walls are not crucial. Different dye samples are tested inside the chamber andpromising results attained. The mixing validation is performed using image processing technique.",
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