Fabrication of alkaline phosphatase biosensor for Hg2+ determination

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A colorimetric biosensor using immobilised alkaline phosphatase (AP) to detect Hg2+ was developed. Copolymer poly(2-hydroxyethyl methacrylate-co-butyl acrylate) (poly(HEMA-co-BA)) were use as matrix to entrap AP. It was discover that incubation time was needed before the mixture of HEMA/BA and AP (in Tis-HCl) undergo UV-initiated photopolymerisation due to hydrophilic and hydrophobic characteristic of monomer HEMA and nBA, respectively. Sensors HB91-AP, HB82-AP and HB73-AP showed a similar optimum pH at pH 8.5. Quantitative determination of Hg2+ using sensor, generate a linear response range from 1 - 5 mM Hg2+(R2=0.9247).

Original languageEnglish
Title of host publication2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings
Pages99-102
Number of pages4
Volume2005
DOIs
Publication statusPublished - 2005
Event2005 Asian Conference on Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research - Kuala Lumpur
Duration: 5 Sep 20057 Sep 2005

Other

Other2005 Asian Conference on Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research
CityKuala Lumpur
Period5/9/057/9/05

Fingerprint

Phosphatases
Biosensors
Fabrication
Photopolymerization
Sensors
Copolymers
Monomers

Keywords

  • Alkaline phosphatase
  • Biosensor
  • Copolymer

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Goh, C. T., Ahmad, M., & Lee, Y. H. (2005). Fabrication of alkaline phosphatase biosensor for Hg2+ determination. In 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings (Vol. 2005, pp. 99-102). [1564515] https://doi.org/10.1109/ASENSE.2005.1564515

Fabrication of alkaline phosphatase biosensor for Hg2+ determination. / Goh, Choo Ta; Ahmad, Musa; Lee, Yook Heng.

2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. Vol. 2005 2005. p. 99-102 1564515.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Goh, CT, Ahmad, M & Lee, YH 2005, Fabrication of alkaline phosphatase biosensor for Hg2+ determination. in 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. vol. 2005, 1564515, pp. 99-102, 2005 Asian Conference on Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research, Kuala Lumpur, 5/9/05. https://doi.org/10.1109/ASENSE.2005.1564515
Goh CT, Ahmad M, Lee YH. Fabrication of alkaline phosphatase biosensor for Hg2+ determination. In 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. Vol. 2005. 2005. p. 99-102. 1564515 https://doi.org/10.1109/ASENSE.2005.1564515
Goh, Choo Ta ; Ahmad, Musa ; Lee, Yook Heng. / Fabrication of alkaline phosphatase biosensor for Hg2+ determination. 2005 Asian Conference on Sensors and the International Conference on New Techniques in Pharmaceutical and Biomedical Research - Proceedings. Vol. 2005 2005. pp. 99-102
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