Fabrication and testing of polydimethylsiloxane (PDMS) microchannel for lab-on-chip (LOC) magnetically-labelled biological cells separation

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Abstract

Microfluidics channel of micron-to millimeter in dimension has been widely used for fluid handling in transporting, mixing and separating biological cells in Lab-on-Chip (LoC) applications. In this research, fabrication and testing of Polydimethylsiloxane (PDMS) microfluidic channel for Lab-on-chip magnetically-labelled biological cells separation is presented. The microchannel is designed with one inlet and outlet. A reservoir or chamber is proposed as an extra component of the microchannel design for ease of trapping the intended biological cells in LoC magnetic separator system. The PDMS microchannel of circular-shaped chamber geometry has been successfully fabricated using replica molding technique from SU-8 negative photoresist mold. An agglomerate-free microbeads flowing has been observed using the fabricated PDMS microchannel. Trapping of microbeads in the trapping chamber with 2.0 A current supply in the continuous microfluidics flow > 100 µL/min has also been demonstrated. In conclusion, a separation of biological cells labelled with magnetic microbeads is expected to be realized using the fabricated PDMS microchannel.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJurnal Teknologi
Volume78
Issue number8-4
DOIs
Publication statusPublished - 2016

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Polydimethylsiloxane
Microchannels
Fabrication
Microfluidics
Testing
Magnetic separators
Photoresists
Molding
Fluids
Geometry

Keywords

  • Biological cells
  • Lab-on-Chip (LoC)
  • Microbeads
  • Microfluidics channel
  • Polydimethylsiloxane (PDMS)

ASJC Scopus subject areas

  • Engineering(all)

Cite this

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title = "Fabrication and testing of polydimethylsiloxane (PDMS) microchannel for lab-on-chip (LOC) magnetically-labelled biological cells separation",
abstract = "Microfluidics channel of micron-to millimeter in dimension has been widely used for fluid handling in transporting, mixing and separating biological cells in Lab-on-Chip (LoC) applications. In this research, fabrication and testing of Polydimethylsiloxane (PDMS) microfluidic channel for Lab-on-chip magnetically-labelled biological cells separation is presented. The microchannel is designed with one inlet and outlet. A reservoir or chamber is proposed as an extra component of the microchannel design for ease of trapping the intended biological cells in LoC magnetic separator system. The PDMS microchannel of circular-shaped chamber geometry has been successfully fabricated using replica molding technique from SU-8 negative photoresist mold. An agglomerate-free microbeads flowing has been observed using the fabricated PDMS microchannel. Trapping of microbeads in the trapping chamber with 2.0 A current supply in the continuous microfluidics flow > 100 µL/min has also been demonstrated. In conclusion, a separation of biological cells labelled with magnetic microbeads is expected to be realized using the fabricated PDMS microchannel.",
keywords = "Biological cells, Lab-on-Chip (LoC), Microbeads, Microfluidics channel, Polydimethylsiloxane (PDMS)",
author = "Ummikalsom Abidin and Jumril Yunas and {Yeop Majlis}, Burhanuddin",
year = "2016",
doi = "10.11113/jt.v78.9587",
language = "English",
volume = "78",
pages = "73--80",
journal = "Jurnal Teknologi",
issn = "0127-9696",
publisher = "Penerbit Universiti Teknologi Malaysia",
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T1 - Fabrication and testing of polydimethylsiloxane (PDMS) microchannel for lab-on-chip (LOC) magnetically-labelled biological cells separation

AU - Abidin, Ummikalsom

AU - Yunas, Jumril

AU - Yeop Majlis, Burhanuddin

PY - 2016

Y1 - 2016

N2 - Microfluidics channel of micron-to millimeter in dimension has been widely used for fluid handling in transporting, mixing and separating biological cells in Lab-on-Chip (LoC) applications. In this research, fabrication and testing of Polydimethylsiloxane (PDMS) microfluidic channel for Lab-on-chip magnetically-labelled biological cells separation is presented. The microchannel is designed with one inlet and outlet. A reservoir or chamber is proposed as an extra component of the microchannel design for ease of trapping the intended biological cells in LoC magnetic separator system. The PDMS microchannel of circular-shaped chamber geometry has been successfully fabricated using replica molding technique from SU-8 negative photoresist mold. An agglomerate-free microbeads flowing has been observed using the fabricated PDMS microchannel. Trapping of microbeads in the trapping chamber with 2.0 A current supply in the continuous microfluidics flow > 100 µL/min has also been demonstrated. In conclusion, a separation of biological cells labelled with magnetic microbeads is expected to be realized using the fabricated PDMS microchannel.

AB - Microfluidics channel of micron-to millimeter in dimension has been widely used for fluid handling in transporting, mixing and separating biological cells in Lab-on-Chip (LoC) applications. In this research, fabrication and testing of Polydimethylsiloxane (PDMS) microfluidic channel for Lab-on-chip magnetically-labelled biological cells separation is presented. The microchannel is designed with one inlet and outlet. A reservoir or chamber is proposed as an extra component of the microchannel design for ease of trapping the intended biological cells in LoC magnetic separator system. The PDMS microchannel of circular-shaped chamber geometry has been successfully fabricated using replica molding technique from SU-8 negative photoresist mold. An agglomerate-free microbeads flowing has been observed using the fabricated PDMS microchannel. Trapping of microbeads in the trapping chamber with 2.0 A current supply in the continuous microfluidics flow > 100 µL/min has also been demonstrated. In conclusion, a separation of biological cells labelled with magnetic microbeads is expected to be realized using the fabricated PDMS microchannel.

KW - Biological cells

KW - Lab-on-Chip (LoC)

KW - Microbeads

KW - Microfluidics channel

KW - Polydimethylsiloxane (PDMS)

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