Fabricating long shelf life potentiometric urea biosensors using modified MWCNTs on screen printed electrodes

Kasra Saeedfar, Yook Heng Lee, Majid Rezayi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new method was used to functionalize multiwalled carbon nanotubes with carboxylic groups (MWCNTCOOH) via simultaneous sonication and UV radiation. MWCNTCOOH modified with urease enzyme enabled immobilization of urease onto the electrode membrane without enzyme leaching. New bio-conjugated materials were deposited onto a Ag/AgCl screen printed electrode (SPE), which contained a layer of non-plasticized acrylate pH-selective membrane functioning as potentiometric urea biosensor. The optimum pH and phosphate buffer concentrations for the urea biosensor were pH 7.0 and 0.1 mM, respectively. The dynamic linear response range was from 5.0×10-5 M to 1.0×10-2 M, and we observed a Nernstian slope of 59.74±0.72 mV/decade (R2 = 0.98, n = 5). The biosensor had a detection limit of 5.0×10-5 M urea. Biosensor reproducibility and repeatability were 2.09% and 1.19% relative standard deviation, respectively (n = 5). The MWCNT-based potentiometric urea biosensor has negligible interference coming from Na+, K+, Ca2+, Mg2+-, and NH+ 4 interfering cations. The biochemically modified MWCNT allowed further improvement of potentiometric urea biosensor performance in terms of response time, linear response range, and long-Term stability.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalSensor Letters
Volume15
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017

Fingerprint

ureas
bioinstrumentation
Biosensors
Urea
life (durability)
Electrodes
electrodes
enzymes
Enzyme immobilization
membranes
Membranes
Sonication
Multiwalled carbon nanotubes (MWCN)
leaching
acrylates
immobilization
Ultraviolet radiation
Leaching
standard deviation
phosphates

Keywords

  • Carbon nanotube
  • Potentiometric method
  • Screen printed electrode
  • Urea biosensor

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

Cite this

Fabricating long shelf life potentiometric urea biosensors using modified MWCNTs on screen printed electrodes. / Saeedfar, Kasra; Lee, Yook Heng; Rezayi, Majid.

In: Sensor Letters, Vol. 15, No. 2, 01.02.2017, p. 97-103.

Research output: Contribution to journalArticle

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