Fabrikasi slaid mikroatur cDNA lates calcarifer

Translated title of the contribution: Fabricalion of lates calcarifer cDNA microarray slide

Khoo Choon Kiat, Adura Mohd-Adnan, Kua Beng Chu, Abdul Munir Abd. Murad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

DNA microarray technology has become an essential part of biological research. It enables the genome-scale analysis of gene expression of various model systems performed. DNA microarray performance is measured by parameters like spot density, spot characteristics (morphology, probe density and hybridised intensity), background, specificity and sensitivity. Using the GeneTac™ G3 arrayer, we fabricated the Asian seabass (Lates calacrifer) cDNA microarray. comprising 1920 selected cDNAs clones sourced from spleen and liver cDNA libraries, and spotted in duplicate. Putative functions encoded by these clones include immunity, stress response, metabolism, transport, transcription and translation. Negative controls such as oligonucleotide (A)50′ and several Saccharomyces cerevisiae and Escherichia coli genes as well as positive controls such as a serial dilution of L. calcarifer genomic DNA and cDNA were also spotted onto the glass slide. It was observed that the probes concentration ranging from 150-250 μg/mL and the type of positive and negative control employed produced quality slide. In addition, the overnight drying of the spotted slides and slide rehydrating produced morphologically even and round DNA spots. Slide quality controls employed demonstrated the functionality of the fabricated slides in profiling the L. calcarifer transcriptome response toward Cryptocaryon irritans infection.

Original languageUndefined/Unknown
Pages (from-to)609-617
Number of pages9
JournalSains Malaysiana
Volume38
Issue number4
Publication statusPublished - Aug 2009

Fingerprint

Lates calcarifer
Lates
clones
microarray technology
DNA
oligonucleotides
transcriptome
cDNA libraries
translation (genetics)
quality control
glass
Saccharomyces cerevisiae
stress response
spleen
transcription (genetics)
immunity
drying
Escherichia coli
genomics
gene expression

Keywords

  • DNA microarray: Lates calcarifer
  • Transcriptome profiling

ASJC Scopus subject areas

  • General

Cite this

Kiat, K. C., Mohd-Adnan, A., Chu, K. B., & Abd. Murad, A. M. (2009). Fabrikasi slaid mikroatur cDNA lates calcarifer. Sains Malaysiana, 38(4), 609-617.

Fabrikasi slaid mikroatur cDNA lates calcarifer. / Kiat, Khoo Choon; Mohd-Adnan, Adura; Chu, Kua Beng; Abd. Murad, Abdul Munir.

In: Sains Malaysiana, Vol. 38, No. 4, 08.2009, p. 609-617.

Research output: Contribution to journalArticle

Kiat, KC, Mohd-Adnan, A, Chu, KB & Abd. Murad, AM 2009, 'Fabrikasi slaid mikroatur cDNA lates calcarifer', Sains Malaysiana, vol. 38, no. 4, pp. 609-617.
Kiat KC, Mohd-Adnan A, Chu KB, Abd. Murad AM. Fabrikasi slaid mikroatur cDNA lates calcarifer. Sains Malaysiana. 2009 Aug;38(4):609-617.
Kiat, Khoo Choon ; Mohd-Adnan, Adura ; Chu, Kua Beng ; Abd. Murad, Abdul Munir. / Fabrikasi slaid mikroatur cDNA lates calcarifer. In: Sains Malaysiana. 2009 ; Vol. 38, No. 4. pp. 609-617.
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