Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis

S. Nor Faizura, K. Farahayu, A. B Mohd Faizal, A. A S Asmahani, R. Amir, N. Nazalan, A. B Farah Diba, M. Nor Muhammad, Abdul Munir Abd. Murad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two preparation methods of crude extracts of psychrophilic yeast Glaciozyma antarctica were compared in order to obtain a good recovery of intracellular proteins. Extraction with mechanical procedures using sonication was found to be more effective for obtaining good yield compare to alkaline treatment method. The procedure is simple, rapid, and produce better yield. A total of 52 proteins were identified by combining both extraction methods. Most of the proteins identified in this study involves in the metabolic process including glycolysis pathway, pentose phosphate pathway, pyruyate decarboxylation and also urea cyle. Several chaperons were identified including probable cpr1-cyclophilin (peptidylprolyl isomerase), macrolide-binding protein fkbp12 and heat shock proteins which were postulate to accelerate proper protein folding. Characteristic of the fundamental cellular processes inferred from the expressedproteome highlight the evolutionary and functional complexity existing in this domain of life.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages264-270
Number of pages7
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

Antarctic regions
proteins
pentose
glycolysis
decarboxylation
yeast
axioms
ureas
folding
phosphates
recovery
shock
heat
preparation

Keywords

  • Cpr1-cyclophilin
  • Glaciozyma antarctica
  • Heat shock proteins
  • Sonication

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Faizura, S. N., Farahayu, K., Faizal, A. B. M., Asmahani, A. A. S., Amir, R., Nazalan, N., ... Abd. Murad, A. M. (2013). Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis. In AIP Conference Proceedings (Vol. 1571, pp. 264-270) https://doi.org/10.1063/1.4858666

Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis. / Faizura, S. Nor; Farahayu, K.; Faizal, A. B Mohd; Asmahani, A. A S; Amir, R.; Nazalan, N.; Diba, A. B Farah; Muhammad, M. Nor; Abd. Murad, Abdul Munir.

AIP Conference Proceedings. Vol. 1571 2013. p. 264-270.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Faizura, SN, Farahayu, K, Faizal, ABM, Asmahani, AAS, Amir, R, Nazalan, N, Diba, ABF, Muhammad, MN & Abd. Murad, AM 2013, Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis. in AIP Conference Proceedings. vol. 1571, pp. 264-270, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858666
Faizura SN, Farahayu K, Faizal ABM, Asmahani AAS, Amir R, Nazalan N et al. Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis. In AIP Conference Proceedings. Vol. 1571. 2013. p. 264-270 https://doi.org/10.1063/1.4858666
Faizura, S. Nor ; Farahayu, K. ; Faizal, A. B Mohd ; Asmahani, A. A S ; Amir, R. ; Nazalan, N. ; Diba, A. B Farah ; Muhammad, M. Nor ; Abd. Murad, Abdul Munir. / Extraction of intracellular protein from Glaciozyma antarctica for proteomics analysis. AIP Conference Proceedings. Vol. 1571 2013. pp. 264-270
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