Extraction of hydrolysable tannins from Phyllanthus niruri Linn. Effects of solvents and extraction methods

Masturah Markom, Masitah Hasan, Wan Ramli Wan Daud, Harcharan Singh, Jamaliah Md Jahim

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Effects of solvent types and extraction methods (solvent extraction (SE), supercritical fluid extraction (SFE) and pressurized water extraction (PWE)) were investigated for effective recovery of bioactive hydrolysable tannins from Phyllanthus niruri Linn. Various organic and aqueous solvents screened by Soxhlet method showed that the gallic acid and ellagic acid contents increased with water content whereas corilagin yield reached a maximum value at 30% (v/v) ethanol in water. At a fixed temperature, solvent extraction by Soxhlet is the best method for gallic and ellagic acid extractions, whereas pressurized methods are better for the corilagin extraction. Even though exhaustive extraction is achieved fastest by PWE, SFE with the addition of ethanol-water cosolvent is superior in terms of low liquid solvent consumption and component fractionation produced. Solvent polarity, solvent-to-solid ratio and contact time play significant roles in determining the most efficient method for tannin extraction.

Original languageEnglish
Pages (from-to)487-496
Number of pages10
JournalSeparation and Purification Technology
Volume52
Issue number3
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Hydrolyzable Tannins
Tannins
Ellagic acid
Ellagic Acid
Gallic Acid
Supercritical fluids
Water
Solvent extraction
Ethanol
Fractionation
Water content

Keywords

  • Ellagitannins
  • Phyllanthus niruri
  • Solvent extraction
  • Sub-critical water
  • Supercritical fluid extraction

ASJC Scopus subject areas

  • Filtration and Separation

Cite this

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abstract = "Effects of solvent types and extraction methods (solvent extraction (SE), supercritical fluid extraction (SFE) and pressurized water extraction (PWE)) were investigated for effective recovery of bioactive hydrolysable tannins from Phyllanthus niruri Linn. Various organic and aqueous solvents screened by Soxhlet method showed that the gallic acid and ellagic acid contents increased with water content whereas corilagin yield reached a maximum value at 30{\%} (v/v) ethanol in water. At a fixed temperature, solvent extraction by Soxhlet is the best method for gallic and ellagic acid extractions, whereas pressurized methods are better for the corilagin extraction. Even though exhaustive extraction is achieved fastest by PWE, SFE with the addition of ethanol-water cosolvent is superior in terms of low liquid solvent consumption and component fractionation produced. Solvent polarity, solvent-to-solid ratio and contact time play significant roles in determining the most efficient method for tannin extraction.",
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AU - Hasan, Masitah

AU - Wan Daud, Wan Ramli

AU - Singh, Harcharan

AU - Md Jahim, Jamaliah

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N2 - Effects of solvent types and extraction methods (solvent extraction (SE), supercritical fluid extraction (SFE) and pressurized water extraction (PWE)) were investigated for effective recovery of bioactive hydrolysable tannins from Phyllanthus niruri Linn. Various organic and aqueous solvents screened by Soxhlet method showed that the gallic acid and ellagic acid contents increased with water content whereas corilagin yield reached a maximum value at 30% (v/v) ethanol in water. At a fixed temperature, solvent extraction by Soxhlet is the best method for gallic and ellagic acid extractions, whereas pressurized methods are better for the corilagin extraction. Even though exhaustive extraction is achieved fastest by PWE, SFE with the addition of ethanol-water cosolvent is superior in terms of low liquid solvent consumption and component fractionation produced. Solvent polarity, solvent-to-solid ratio and contact time play significant roles in determining the most efficient method for tannin extraction.

AB - Effects of solvent types and extraction methods (solvent extraction (SE), supercritical fluid extraction (SFE) and pressurized water extraction (PWE)) were investigated for effective recovery of bioactive hydrolysable tannins from Phyllanthus niruri Linn. Various organic and aqueous solvents screened by Soxhlet method showed that the gallic acid and ellagic acid contents increased with water content whereas corilagin yield reached a maximum value at 30% (v/v) ethanol in water. At a fixed temperature, solvent extraction by Soxhlet is the best method for gallic and ellagic acid extractions, whereas pressurized methods are better for the corilagin extraction. Even though exhaustive extraction is achieved fastest by PWE, SFE with the addition of ethanol-water cosolvent is superior in terms of low liquid solvent consumption and component fractionation produced. Solvent polarity, solvent-to-solid ratio and contact time play significant roles in determining the most efficient method for tannin extraction.

KW - Ellagitannins

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KW - Sub-critical water

KW - Supercritical fluid extraction

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