Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions

Norazelina Sah Mohd Ismail, Nazaruddin Ramli, Norziah Mohd Hani, Zainudin Meon

    Research output: Contribution to journalArticle

    53 Citations (Scopus)

    Abstract

    The extraction of pectin from dragon fruit (Hylocereus polyrhizus) peels under three different extraction conditions was identified as an alternative source of commercial pectin. In this work, dried alcohol-insoluble residues (AIR) of dragon fruit peels were treated separately with 0.25% ammonium oxalate/oxalic acid at a pH of 4.6 at 85°C; 0.03 M HCl at a pH of 1.5 at 85°C; and de-ionized water at 75°C. The pectin obtained from these methods was compared in terms of yield, physicochemical properties and chemical structure. Fourier Transform Infrared Spectroscopy (FTIR) was used in the identification of dragon fruit pectins. The results showed that the pectin yield (14.96-20.14% based on dry weight), moisture content (11.13-11.33%), ash content (6.88-11.55%), equivalent weight (475.64-713.99), methoxyl content (2.98-4.34%), anhydrouronic acid (45.25-52.45%) and the degree of esterification (31.05-46.96%) varied significantly (p < 0.05) with the various extraction conditions used. Pectin extracted with ammonium oxalate gave the highest yield of pectin, with high purity and low ash content. Based on the value of methoxyl content and the degree of esterification, dragon fruit pectin can be categorized as low-methoxyl pectin.

    Original languageEnglish
    Pages (from-to)41-45
    Number of pages5
    JournalSains Malaysiana
    Volume41
    Issue number1
    Publication statusPublished - Jan 2012

    Fingerprint

    Hylocereus polyrhizus
    Hylocereus
    pectins
    ammonium oxalate
    esterification
    ash content
    fruit peels
    oxalic acid
    Fourier transform infrared spectroscopy
    chemical structure
    purity
    physicochemical properties
    alcohols

    Keywords

    • Alcohol insoluble residue
    • Dragon fruit
    • Extraction conditions
    • Pectin

    ASJC Scopus subject areas

    • General

    Cite this

    Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions. / Ismail, Norazelina Sah Mohd; Ramli, Nazaruddin; Hani, Norziah Mohd; Meon, Zainudin.

    In: Sains Malaysiana, Vol. 41, No. 1, 01.2012, p. 41-45.

    Research output: Contribution to journalArticle

    Ismail, Norazelina Sah Mohd ; Ramli, Nazaruddin ; Hani, Norziah Mohd ; Meon, Zainudin. / Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions. In: Sains Malaysiana. 2012 ; Vol. 41, No. 1. pp. 41-45.
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    abstract = "The extraction of pectin from dragon fruit (Hylocereus polyrhizus) peels under three different extraction conditions was identified as an alternative source of commercial pectin. In this work, dried alcohol-insoluble residues (AIR) of dragon fruit peels were treated separately with 0.25{\%} ammonium oxalate/oxalic acid at a pH of 4.6 at 85°C; 0.03 M HCl at a pH of 1.5 at 85°C; and de-ionized water at 75°C. The pectin obtained from these methods was compared in terms of yield, physicochemical properties and chemical structure. Fourier Transform Infrared Spectroscopy (FTIR) was used in the identification of dragon fruit pectins. The results showed that the pectin yield (14.96-20.14{\%} based on dry weight), moisture content (11.13-11.33{\%}), ash content (6.88-11.55{\%}), equivalent weight (475.64-713.99), methoxyl content (2.98-4.34{\%}), anhydrouronic acid (45.25-52.45{\%}) and the degree of esterification (31.05-46.96{\%}) varied significantly (p < 0.05) with the various extraction conditions used. Pectin extracted with ammonium oxalate gave the highest yield of pectin, with high purity and low ash content. Based on the value of methoxyl content and the degree of esterification, dragon fruit pectin can be categorized as low-methoxyl pectin.",
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