Extraction and characterisation of proteins from a Malaysian Isolate of Magnaporthe grisea

Hari Kumar Krishnan, Kalaivani K. Nadarajah, Ilakiya Sharanee Kumar, Vaanee Sangappilai

Research output: Contribution to journalArticle

Abstract

Aims: Rice blast, a disease caused by the fungus Magnaporthe grisea is one of the serious diseases of rice in the world. The main objective of this study is to isolate and characterise the proteins extracted from the rice blast fungus, M. grisea 7.6. Methodology and results: Through comparative 2-D analyses of the crude protein extracts obtained from this fungus, we were able to identify 88 protein spots through MALDI-TOF. These proteins were then classified into 8 functional groups through the Pfam and KEGG databases into hypothetical, transferases, energy and carbon metabolism, oxidoreductases, molecular chaperone, hydrolases, structural organisation and kinases. The individual protein's functions were then identified and their possible role in pathogenesis, virulence and proliferation of M. grisea 7.6 were predicted. Conclusion, significance and impact of study: Through the assays conducted, we were able to identify some proteins and pathways that could be targeted in developing fungicides and used in future mutagenesis studies.

Original languageEnglish
Pages (from-to)519-533
Number of pages15
JournalMalaysian Journal of Microbiology
Volume14
Issue numberSpecialissue6
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Magnaporthe
Proteins
Fungi
Molecular Chaperones
Mycoses
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Hydrolases
Transferases
Complex Mixtures
Mutagenesis
Energy Metabolism
Virulence
Oxidoreductases
Phosphotransferases
Carbon
Databases

Keywords

  • Elicitor
  • Magnaporthe grisea
  • Proliferation
  • Protein
  • Stress

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Extraction and characterisation of proteins from a Malaysian Isolate of Magnaporthe grisea. / Krishnan, Hari Kumar; K. Nadarajah, Kalaivani; Sharanee Kumar, Ilakiya; Sangappilai, Vaanee.

In: Malaysian Journal of Microbiology, Vol. 14, No. Specialissue6, 01.01.2018, p. 519-533.

Research output: Contribution to journalArticle

Krishnan, Hari Kumar ; K. Nadarajah, Kalaivani ; Sharanee Kumar, Ilakiya ; Sangappilai, Vaanee. / Extraction and characterisation of proteins from a Malaysian Isolate of Magnaporthe grisea. In: Malaysian Journal of Microbiology. 2018 ; Vol. 14, No. Specialissue6. pp. 519-533.
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