Expressions of P-glycoprotein in treatment-Resistant Helicobacter pylori patients

Marhanis Salihah Omar, Andrew Crowe, Chin Yen Tay, Jeffery Hughes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: There is an increasing incidence of Helicobacter pylori eradication therapy failure secondary to antibiotic resistance. In this study, we aimed to assess the P-glycoprotein expression levels among subjects who were H. pylori-positive and received multiple courses of eradication therapy to understand factors that may associate with their non-responses. Methods: Eleven resistant subjects were recruited during their hospital visit for upper gastrointestinal endoscopies. H. pylori infection status was confirmed by rapid urease test and bacterial culture. P-glycoprotein expressions from the antral and duodenal biopsies were measured by Western blot. The data was compared with H. pylori-negative (n = 54) and H. pylori-positive-treatment naïve (n = 22) that were recruited earlier in another study. Results: The resistant group showed higher relative antral P-glycoprotein expression compared to the H. pylori-negative group ( p = 0.0361). Most subjects demonstrated resistance to clarithromycin (72%), metronidazole (63.6%) or both (54.5%). No significant difference was observed between the P-glycoprotein expression in H. pylori-treatment resistant and H. pylori-positive-treatment naïve groups ( p = 0.319). Conclusion: H. pylori infection induces the expression of P-glycoprotein in the antrum. This study shows a potential protective mechanism which may be exploited through the use of Pglycoprotein inducers, such as rifabutin, may be beneficial in eradication regimens.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalJournal of Applied Biomedicine
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Nov 2014

Fingerprint

Helicobacter pylori
P-Glycoprotein
Rifabutin
Therapeutics
Helicobacter Infections
Endoscopy
Clarithromycin
Urease
Biopsy
Metronidazole
gastrointestinal endoscopy
clarithromycin
P-glycoproteins
metronidazole
therapeutics
Anti-Bacterial Agents
Gastrointestinal Endoscopy
urease
Microbial Drug Resistance
antibiotic resistance

Keywords

  • ABCB1
  • Antibiotic resistance
  • Gastrointestinal tract
  • Peptic ulcer disease
  • Protein expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Expressions of P-glycoprotein in treatment-Resistant Helicobacter pylori patients. / Omar, Marhanis Salihah; Crowe, Andrew; Tay, Chin Yen; Hughes, Jeffery.

In: Journal of Applied Biomedicine, Vol. 12, No. 4, 01.11.2014, p. 263-269.

Research output: Contribution to journalArticle

Omar, Marhanis Salihah ; Crowe, Andrew ; Tay, Chin Yen ; Hughes, Jeffery. / Expressions of P-glycoprotein in treatment-Resistant Helicobacter pylori patients. In: Journal of Applied Biomedicine. 2014 ; Vol. 12, No. 4. pp. 263-269.
@article{d53ebb98ce2a462a8f6d4c55fbee8adc,
title = "Expressions of P-glycoprotein in treatment-Resistant Helicobacter pylori patients",
abstract = "Objective: There is an increasing incidence of Helicobacter pylori eradication therapy failure secondary to antibiotic resistance. In this study, we aimed to assess the P-glycoprotein expression levels among subjects who were H. pylori-positive and received multiple courses of eradication therapy to understand factors that may associate with their non-responses. Methods: Eleven resistant subjects were recruited during their hospital visit for upper gastrointestinal endoscopies. H. pylori infection status was confirmed by rapid urease test and bacterial culture. P-glycoprotein expressions from the antral and duodenal biopsies were measured by Western blot. The data was compared with H. pylori-negative (n = 54) and H. pylori-positive-treatment na{\"i}ve (n = 22) that were recruited earlier in another study. Results: The resistant group showed higher relative antral P-glycoprotein expression compared to the H. pylori-negative group ( p = 0.0361). Most subjects demonstrated resistance to clarithromycin (72{\%}), metronidazole (63.6{\%}) or both (54.5{\%}). No significant difference was observed between the P-glycoprotein expression in H. pylori-treatment resistant and H. pylori-positive-treatment na{\"i}ve groups ( p = 0.319). Conclusion: H. pylori infection induces the expression of P-glycoprotein in the antrum. This study shows a potential protective mechanism which may be exploited through the use of Pglycoprotein inducers, such as rifabutin, may be beneficial in eradication regimens.",
keywords = "ABCB1, Antibiotic resistance, Gastrointestinal tract, Peptic ulcer disease, Protein expression",
author = "Omar, {Marhanis Salihah} and Andrew Crowe and Tay, {Chin Yen} and Jeffery Hughes",
year = "2014",
month = "11",
day = "1",
doi = "10.1016/j.jab.2014.02.001",
language = "English",
volume = "12",
pages = "263--269",
journal = "Journal of Applied Biomedicine",
issn = "1214-021X",
publisher = "University of South Bohemia",
number = "4",

}

TY - JOUR

T1 - Expressions of P-glycoprotein in treatment-Resistant Helicobacter pylori patients

AU - Omar, Marhanis Salihah

AU - Crowe, Andrew

AU - Tay, Chin Yen

AU - Hughes, Jeffery

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Objective: There is an increasing incidence of Helicobacter pylori eradication therapy failure secondary to antibiotic resistance. In this study, we aimed to assess the P-glycoprotein expression levels among subjects who were H. pylori-positive and received multiple courses of eradication therapy to understand factors that may associate with their non-responses. Methods: Eleven resistant subjects were recruited during their hospital visit for upper gastrointestinal endoscopies. H. pylori infection status was confirmed by rapid urease test and bacterial culture. P-glycoprotein expressions from the antral and duodenal biopsies were measured by Western blot. The data was compared with H. pylori-negative (n = 54) and H. pylori-positive-treatment naïve (n = 22) that were recruited earlier in another study. Results: The resistant group showed higher relative antral P-glycoprotein expression compared to the H. pylori-negative group ( p = 0.0361). Most subjects demonstrated resistance to clarithromycin (72%), metronidazole (63.6%) or both (54.5%). No significant difference was observed between the P-glycoprotein expression in H. pylori-treatment resistant and H. pylori-positive-treatment naïve groups ( p = 0.319). Conclusion: H. pylori infection induces the expression of P-glycoprotein in the antrum. This study shows a potential protective mechanism which may be exploited through the use of Pglycoprotein inducers, such as rifabutin, may be beneficial in eradication regimens.

AB - Objective: There is an increasing incidence of Helicobacter pylori eradication therapy failure secondary to antibiotic resistance. In this study, we aimed to assess the P-glycoprotein expression levels among subjects who were H. pylori-positive and received multiple courses of eradication therapy to understand factors that may associate with their non-responses. Methods: Eleven resistant subjects were recruited during their hospital visit for upper gastrointestinal endoscopies. H. pylori infection status was confirmed by rapid urease test and bacterial culture. P-glycoprotein expressions from the antral and duodenal biopsies were measured by Western blot. The data was compared with H. pylori-negative (n = 54) and H. pylori-positive-treatment naïve (n = 22) that were recruited earlier in another study. Results: The resistant group showed higher relative antral P-glycoprotein expression compared to the H. pylori-negative group ( p = 0.0361). Most subjects demonstrated resistance to clarithromycin (72%), metronidazole (63.6%) or both (54.5%). No significant difference was observed between the P-glycoprotein expression in H. pylori-treatment resistant and H. pylori-positive-treatment naïve groups ( p = 0.319). Conclusion: H. pylori infection induces the expression of P-glycoprotein in the antrum. This study shows a potential protective mechanism which may be exploited through the use of Pglycoprotein inducers, such as rifabutin, may be beneficial in eradication regimens.

KW - ABCB1

KW - Antibiotic resistance

KW - Gastrointestinal tract

KW - Peptic ulcer disease

KW - Protein expression

UR - http://www.scopus.com/inward/record.url?scp=84961367998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961367998&partnerID=8YFLogxK

U2 - 10.1016/j.jab.2014.02.001

DO - 10.1016/j.jab.2014.02.001

M3 - Article

VL - 12

SP - 263

EP - 269

JO - Journal of Applied Biomedicine

JF - Journal of Applied Biomedicine

SN - 1214-021X

IS - 4

ER -