Profil pengekspresan gen mengekod delta 6 dan delta 12-asid lemak desaturase Cunninghamella bainieri semasa biosintesis asid gamma-linolenik

Translated title of the contribution: Expression profiles of delta 6 and delta 12-fatty acid desaturase encoding genes Cunninghamella bainieri during gamma-linoleneic acid biosynthesis

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Abstract

Delta 6-fatty acid desaturase and delta12-fatty acid desaturase are enzymes required for the desaturation steps during gamma-linolenic acid (GLA) biosynthesis in oleaginous fungi. The objectives of this study were to analyse the expression profiles of genes which encode for the delta 6-fatty acid desaturase (des6) and delta 12-fatty acid desaturase (des12) from an oleaginous fungus, Cunninghamella bainieri, during GLA production. Partial gene sequences with the size of 1372 bp for des6 and 1008 bp for des12, were isolated from C. Bainieri. Gene expression analyses via real-time quantitative PCR (RT-qPCR) indicate that the changes in the expression profile of des6 is higher during GLA production as compared to the expression profile of des12. The expression of des6 is the highest after 24 h of growth in the GLA-inducing medium. However, its expression showed a decreasing pattern until 96 h of growth but increased at 120 h. The expression profile of des12 is more consistent with the highest expression levels detected at 120 h of growth. GLA production analysis showed that the amount of GLA in the cell is correlated with the expression of des6. The results of this study suggested that the expression of des6 plays an important role in determining GLA production in C. bainieri.

Original languageMalay
Pages (from-to)1443-1451
Number of pages9
JournalSains Malaysiana
Volume45
Issue number10
Publication statusPublished - 1 Oct 2016

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Cunninghamella
gamma-linolenic acid
stearoyl-CoA desaturase
biosynthesis
acids
oleaginous fungi
genes
quantitative polymerase chain reaction
nucleotide sequences
gene expression

ASJC Scopus subject areas

  • General

Cite this

@article{20780d42f0b4499e90d08da25626d068,
title = "Profil pengekspresan gen mengekod delta 6 dan delta 12-asid lemak desaturase Cunninghamella bainieri semasa biosintesis asid gamma-linolenik",
abstract = "Delta 6-fatty acid desaturase and delta12-fatty acid desaturase are enzymes required for the desaturation steps during gamma-linolenic acid (GLA) biosynthesis in oleaginous fungi. The objectives of this study were to analyse the expression profiles of genes which encode for the delta 6-fatty acid desaturase (des6) and delta 12-fatty acid desaturase (des12) from an oleaginous fungus, Cunninghamella bainieri, during GLA production. Partial gene sequences with the size of 1372 bp for des6 and 1008 bp for des12, were isolated from C. Bainieri. Gene expression analyses via real-time quantitative PCR (RT-qPCR) indicate that the changes in the expression profile of des6 is higher during GLA production as compared to the expression profile of des12. The expression of des6 is the highest after 24 h of growth in the GLA-inducing medium. However, its expression showed a decreasing pattern until 96 h of growth but increased at 120 h. The expression profile of des12 is more consistent with the highest expression levels detected at 120 h of growth. GLA production analysis showed that the amount of GLA in the cell is correlated with the expression of des6. The results of this study suggested that the expression of des6 plays an important role in determining GLA production in C. bainieri.",
keywords = "Cunninghamella bainieri, Delta 12-fatty acid desaturase, Delta 6-fatty acid desaturase, Gamma linoleneic acid, Oleaginous fungi",
author = "Karim, {Noor Adila Abdul} and Zamri Zainal and {Abdul Hamid}, Aidil and {Abu Bakar}, {Farah Diba} and {Abd. Murad}, {Abdul Munir}",
year = "2016",
month = "10",
day = "1",
language = "Malay",
volume = "45",
pages = "1443--1451",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "10",

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TY - JOUR

T1 - Profil pengekspresan gen mengekod delta 6 dan delta 12-asid lemak desaturase Cunninghamella bainieri semasa biosintesis asid gamma-linolenik

AU - Karim, Noor Adila Abdul

AU - Zainal, Zamri

AU - Abdul Hamid, Aidil

AU - Abu Bakar, Farah Diba

AU - Abd. Murad, Abdul Munir

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Delta 6-fatty acid desaturase and delta12-fatty acid desaturase are enzymes required for the desaturation steps during gamma-linolenic acid (GLA) biosynthesis in oleaginous fungi. The objectives of this study were to analyse the expression profiles of genes which encode for the delta 6-fatty acid desaturase (des6) and delta 12-fatty acid desaturase (des12) from an oleaginous fungus, Cunninghamella bainieri, during GLA production. Partial gene sequences with the size of 1372 bp for des6 and 1008 bp for des12, were isolated from C. Bainieri. Gene expression analyses via real-time quantitative PCR (RT-qPCR) indicate that the changes in the expression profile of des6 is higher during GLA production as compared to the expression profile of des12. The expression of des6 is the highest after 24 h of growth in the GLA-inducing medium. However, its expression showed a decreasing pattern until 96 h of growth but increased at 120 h. The expression profile of des12 is more consistent with the highest expression levels detected at 120 h of growth. GLA production analysis showed that the amount of GLA in the cell is correlated with the expression of des6. The results of this study suggested that the expression of des6 plays an important role in determining GLA production in C. bainieri.

AB - Delta 6-fatty acid desaturase and delta12-fatty acid desaturase are enzymes required for the desaturation steps during gamma-linolenic acid (GLA) biosynthesis in oleaginous fungi. The objectives of this study were to analyse the expression profiles of genes which encode for the delta 6-fatty acid desaturase (des6) and delta 12-fatty acid desaturase (des12) from an oleaginous fungus, Cunninghamella bainieri, during GLA production. Partial gene sequences with the size of 1372 bp for des6 and 1008 bp for des12, were isolated from C. Bainieri. Gene expression analyses via real-time quantitative PCR (RT-qPCR) indicate that the changes in the expression profile of des6 is higher during GLA production as compared to the expression profile of des12. The expression of des6 is the highest after 24 h of growth in the GLA-inducing medium. However, its expression showed a decreasing pattern until 96 h of growth but increased at 120 h. The expression profile of des12 is more consistent with the highest expression levels detected at 120 h of growth. GLA production analysis showed that the amount of GLA in the cell is correlated with the expression of des6. The results of this study suggested that the expression of des6 plays an important role in determining GLA production in C. bainieri.

KW - Cunninghamella bainieri

KW - Delta 12-fatty acid desaturase

KW - Delta 6-fatty acid desaturase

KW - Gamma linoleneic acid

KW - Oleaginous fungi

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