Expression profile of immune-related genes in Lates calcarifer infected by Cryptocaryon irritans.

Norashikin Mohd-Shaharuddin, Adura Mohd-Adnan, Beng Chu Kua, Sheila Nathan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cryptocaryon irritans causes Cyptocaryonosis or white spot disease in a wide range of marine fish including Lates calcarifer (Asian seabass). However, the immune response of this fish to the parasite is still poorly understood. In this study, quantitative polymerase chain reaction (qPCR) was performed to assess the expression profile of immune-related genes in L. calcarifer infected by C. irritans. A total of 21 immune-related genes encoding various functions in the fish immune system were utilized for the qPCR analysis. The experiment was initiated with the infection of juvenile fish by exposure to theronts from 200 C. irritans cysts, and non-infected juvenile fish were used as controls. Spleen, liver, gills and kidney tissues were harvested at three days post-infection from control and infected fish. In addition, organs were also harvested on day-10 post-infection from fish that had been allowed to recover from day-4 up to day-10 post-infection. L. calcarifer exhibited pathological changes on day-3 post-infection with the characteristic presence of white spots on the entire fish body, excessive mucus production and formation of a flap over the fish eye. High quality total RNA was extracted from all tissues and qPCR was performed. The qPCR analysis on the cohort of 21 immune-related genes of the various organs harvested on day-3 post-infection demonstrated that most genes were induced significantly (p < 0.05) in all tissues, particularly liver (11/21 genes) and kidney (11/21). The expression profile demonstrated that induction of the MHC Class IIα gene was the highest compared to the other genes followed by serum amyloid A, CC chemokine and hepcidin-2 precursor genes. In fish that were allowed to recover from the C. irritans infection (10 days post-infection), expression of the immune-related genes was down-regulated to levels similar to the control fish. These results provide insights into the interaction between C. irritans and L. calcarifer and suggest that the innate immune system plays an important role in early defence against parasite infection allowing the fish to eventually recover from the infection.

Original languageEnglish
Pages (from-to)762-769
Number of pages8
JournalFish & shellfish immunology
Volume34
Issue number3
DOIs
Publication statusPublished - Mar 2013

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Lates calcarifer
Fish
Fishes
Genes
gene
fish
infection
genes
Polymerase chain reaction
Infection
polymerase chain reaction
quantitative polymerase chain reaction
Polymerase Chain Reaction
Immune system
Dental Caries
immune system
Tissue
Liver
Immune System
parasite

ASJC Scopus subject areas

  • Medicine(all)

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Expression profile of immune-related genes in Lates calcarifer infected by Cryptocaryon irritans. / Mohd-Shaharuddin, Norashikin; Mohd-Adnan, Adura; Kua, Beng Chu; Nathan, Sheila.

In: Fish & shellfish immunology, Vol. 34, No. 3, 03.2013, p. 762-769.

Research output: Contribution to journalArticle

Mohd-Shaharuddin, Norashikin ; Mohd-Adnan, Adura ; Kua, Beng Chu ; Nathan, Sheila. / Expression profile of immune-related genes in Lates calcarifer infected by Cryptocaryon irritans. In: Fish & shellfish immunology. 2013 ; Vol. 34, No. 3. pp. 762-769.
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