Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress

Nur Athirah Yusof, Clemente Michael Vui Ling Wong, Abdul Munir Abdul Murad, Farah Diba Abu Bakar, Nor Muhammad Mahadi, Ahmad Yamin Abdul Rahman, Nursyafiqi Zainuddin, Mohd Nazalan Mohd Najimudin

Research output: Contribution to journalArticle

Abstract

Microbes living in the polar regions have some common and unique strategies to respond to thermal stress. Nevertheless, the amount of information available, especially at the molecular level is lacking for some organisms such as Antarctic psychrophilic yeast. For instance, it is not known whether molecular chaperones in Antarctic yeasts play similar roles to those from mesophilic yeasts when they are exposed to heat stress. Therefore, this project aimed to determine the gene expression patterns and roles of molecular chaperones in Antarctic psychrophilic Glaciozyma antarctica PI12 that was exposed to heat stress. G. antarctica PI12 was grown at its optimal growth temperature of 12ºC and later exposed to heat stresses at 16ºC and 20ºC for 6 hours. Transcriptomes of those cells were extracted, sequenced and analyzed. Thirty-three molecular chaperone genes demonstrated differential expression of which 23 were up-regulated while 10 were down-regulated. Functions of up-regulated molecular chaperone genes were related to protein binding, response to a stimulus, chaperone binding, cellular response to stress, oxidation, and reduction, ATP binding, DNA-damage response and regulation for cellular protein metabolic process. On the other hand, functions of down-regulated molecular chaperone genes were related to chaperone-mediated protein complex assembly, transcription, cellular macromolecule metabolic process, regulation of cell growth and ribosome biogenesis. The findings provided information on how molecular chaperones work together in a complex network to protect the cells under heat stress. It also highlights the evolutionary conserved protective role of molecular chaperones in psychrophilic yeast, G. antarctica, and mesophilic yeast, Saccharomyces cerevisiae.

Original languageEnglish
Pages (from-to)273-292
Number of pages20
JournalPolish Polar Research
Volume40
Issue number3
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

molecular chaperones
Antarctica
yeast
heat stress
yeasts
gene
genes
protein
Polar Regions
polar region
thermal stress
protein binding
gene expression
ribosomes
transcriptome
DNA damage
cell growth
Saccharomyces cerevisiae
stress response
proteins

Keywords

  • Antarctic
  • Heat-stress
  • Molecular chaperone
  • Psychrophile
  • Transcriptome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress. / Yusof, Nur Athirah; Wong, Clemente Michael Vui Ling; Murad, Abdul Munir Abdul; Abu Bakar, Farah Diba; Mahadi, Nor Muhammad; Rahman, Ahmad Yamin Abdul; Zainuddin, Nursyafiqi; Najimudin, Mohd Nazalan Mohd.

In: Polish Polar Research, Vol. 40, No. 3, 01.01.2019, p. 273-292.

Research output: Contribution to journalArticle

Yusof, Nur Athirah ; Wong, Clemente Michael Vui Ling ; Murad, Abdul Munir Abdul ; Abu Bakar, Farah Diba ; Mahadi, Nor Muhammad ; Rahman, Ahmad Yamin Abdul ; Zainuddin, Nursyafiqi ; Najimudin, Mohd Nazalan Mohd. / Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress. In: Polish Polar Research. 2019 ; Vol. 40, No. 3. pp. 273-292.
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AU - Murad, Abdul Munir Abdul

AU - Abu Bakar, Farah Diba

AU - Mahadi, Nor Muhammad

AU - Rahman, Ahmad Yamin Abdul

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