Expression of the Capsicuum annum (Chili) defensin gene in transgenic tomatoes confers enhanced resistance to fungal pathogens

Zamri Zainal, E. Marouf, Ismanizan Ismail, C. K. Fei

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Plant defensins are a group of pathogenesis-related defense mechanism proteins. Transgenic tomato plants expressing the chili defensin gene under the control of a Cauliflower Mosaic Virus 35S (CaMV 35S) promoter were generated. The 5 kDa peptide, corresponding to the chili defensin protein was detected in the total protein fraction extracted from the transgenic plants. When compared to partially-purified peptide extracts from untransformed tomato plants, those from transgenic plant possessed the ability to reduce the growth of several fungi in vitro. T 2 transgenic plants were selected and tested for resistance against Fusarium sp. and Phytophthora infestans. The transgenie lines were more resistant to infection by these pathogens than the control plants.

Original languageEnglish
Pages (from-to)70-79
Number of pages10
JournalAmerican Journal of Plant Physiology
Volume4
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Defensins
Genetically Modified Plants
Lycopersicon esculentum
genetically modified organisms
tomatoes
transgenic plants
pathogens
Genes
genes
Phytophthora infestans
peptides
Caulimovirus
Peptides
Proteins
proteins
Cauliflower mosaic virus
Fusarium
Infection Control
defense mechanisms
Fungi

Keywords

  • Agrobacterium tumefaciens
  • Antifungal activity
  • Disease resistance
  • Fusarium sp.
  • Lycopersicon esculentum
  • Phytophthora infestans

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

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abstract = "Plant defensins are a group of pathogenesis-related defense mechanism proteins. Transgenic tomato plants expressing the chili defensin gene under the control of a Cauliflower Mosaic Virus 35S (CaMV 35S) promoter were generated. The 5 kDa peptide, corresponding to the chili defensin protein was detected in the total protein fraction extracted from the transgenic plants. When compared to partially-purified peptide extracts from untransformed tomato plants, those from transgenic plant possessed the ability to reduce the growth of several fungi in vitro. T 2 transgenic plants were selected and tested for resistance against Fusarium sp. and Phytophthora infestans. The transgenie lines were more resistant to infection by these pathogens than the control plants.",
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AU - Zainal, Zamri

AU - Marouf, E.

AU - Ismail, Ismanizan

AU - Fei, C. K.

PY - 2009

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