Expression of p53, bcl-2 and Ki-67 in cervical intraepithelial neoplasia and invasive squamous cell carcinoma of the uterine cervix

Mee Lee Looi, Ahmad Zailani Hatta Mohd Dali, Siti Aishah Md Ali, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE: To assess the expression of p53, bcl-2 and Ki-67 in the progression of cervical neoplasia. STUDY DESIGN: A total of 131 cervical specimens, consisting of normal cervical epithelium (n = 43), cervical intraepithelial neoplasia (CIN) lesions (n = 40) and cervical squamous cell carcinomas (SCCs) (n = 48) were examined immunohistochemically in paraffin sections for expression of p53, bcl-2 and Ki-67. RESULTS: Immunoreactivity of p53 was found in 27% of SCC cases, but it had no significant relationship with SCC staging (p=0.791). Immunoreactivity of bcl-2 was observed in 33% of CIN 3 cases. We found a significant relationship (χ2 test: p = 0.009) between the expression of bcl-2 and CIN grading. Ki-67 index was higher in high grade CIN (HGCIN: CIN 2 and 3) and SCC lesions compared to normal cervices. Ki-67 index showed a correlation with bcl-2 protein expression (p = 0.030), but not with p53 protein expression (p = 0.239). CONCLUSION: HGCIN is an early stage to demonstrate the alteration of bcl-2 and Ki-67 expressions. Progression of neoplasia in the uterine cervix is accompanied by an increase of antiapoptotic protein, bcl-2 as well as cellular proliferation.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalAnalytical and Quantitative Cytology and Histology
Volume30
Issue number2
Publication statusPublished - Apr 2008

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Cervical Intraepithelial Neoplasia
Cervix Uteri
Squamous Cell Carcinoma
Proteins
Paraffin
Neoplasms
Epithelium
Cell Proliferation

Keywords

  • Bcl-2
  • Cervical intraepithelial neoplasia
  • Ki-67
  • p53
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Cell Biology

Cite this

Expression of p53, bcl-2 and Ki-67 in cervical intraepithelial neoplasia and invasive squamous cell carcinoma of the uterine cervix. / Looi, Mee Lee; Mohd Dali, Ahmad Zailani Hatta; Ali, Siti Aishah Md; Wan Ngah, Wan Zurinah; Mohd Yusof, Yasmin Anum.

In: Analytical and Quantitative Cytology and Histology, Vol. 30, No. 2, 04.2008, p. 63-70.

Research output: Contribution to journalArticle

Looi, Mee Lee ; Mohd Dali, Ahmad Zailani Hatta ; Ali, Siti Aishah Md ; Wan Ngah, Wan Zurinah ; Mohd Yusof, Yasmin Anum. / Expression of p53, bcl-2 and Ki-67 in cervical intraepithelial neoplasia and invasive squamous cell carcinoma of the uterine cervix. In: Analytical and Quantitative Cytology and Histology. 2008 ; Vol. 30, No. 2. pp. 63-70.
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AU - Wan Ngah, Wan Zurinah

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AB - OBJECTIVE: To assess the expression of p53, bcl-2 and Ki-67 in the progression of cervical neoplasia. STUDY DESIGN: A total of 131 cervical specimens, consisting of normal cervical epithelium (n = 43), cervical intraepithelial neoplasia (CIN) lesions (n = 40) and cervical squamous cell carcinomas (SCCs) (n = 48) were examined immunohistochemically in paraffin sections for expression of p53, bcl-2 and Ki-67. RESULTS: Immunoreactivity of p53 was found in 27% of SCC cases, but it had no significant relationship with SCC staging (p=0.791). Immunoreactivity of bcl-2 was observed in 33% of CIN 3 cases. We found a significant relationship (χ2 test: p = 0.009) between the expression of bcl-2 and CIN grading. Ki-67 index was higher in high grade CIN (HGCIN: CIN 2 and 3) and SCC lesions compared to normal cervices. Ki-67 index showed a correlation with bcl-2 protein expression (p = 0.030), but not with p53 protein expression (p = 0.239). CONCLUSION: HGCIN is an early stage to demonstrate the alteration of bcl-2 and Ki-67 expressions. Progression of neoplasia in the uterine cervix is accompanied by an increase of antiapoptotic protein, bcl-2 as well as cellular proliferation.

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