Expression of multidrug resistance (MDR) proteins and in vitro drug resistance in acute leukemias

Fazlina Nordin, A. Maha, A. Rahman A. Jamal, Zarina Abdul Latiff, S. K. Cheong, H. Hamidah, O. Ainoon, Syed Zulkifli Syed Zakaria, Noor Hamidah Hussin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The expression of the multidrug resistance (MDR) proteins may influence the outcome of treatment in patients with acute leukemia. The aim of this study was to determine the IC 50 of cytotoxic drugs (cytosine arabinoside, ara-C and daunorubicin, dnr) using the in vitro-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)2H-tetrazolium, inner salt (MTS) assay method. A total of 82 newly diagnosed acute leukemia cases (43 adult myeloid leukaemia, AML cases and 39 acute lymphoblastic leukaemia, ALL cases) and 16 relapsed cases (8 AML cases and 8 ALL cases) were studied. The MTS assay was performed using two cytotoxic drugs, dnr and ara-C. Cells were incubated with different concentrations of drugs for 4 days and the IC 50 was extrapolated from the viability curve. In newly diagnosed cases, we found that childhood ALL samples showed higher IC 50 values of dnr (0.040 ± 2.320) compared to adult AML samples (0.021 ± 0.158). In contrast, newly diagnosed adult AML samples showed higher IC 50 values of ara-C (0.157 ± 0.529) compared to childhood ALL samples (0.100 ± 2.350). In relapsed cases, two samples of childhood ALL showed IC 50 values of dnr (0.910 ± 1.760) and ara-C (1.310 ± 2.390), which was higher compared to childhood AML samples (0.129 ± 0.214 and 0.210 ± 0.003, respectively). However, there was no correlation between IC 50 values of these drugs tested with clinical outcome. In conclusion, we found that MTS assay is an easy, rapid and non laborious method to study in vitro drug resistance in acute leukaemia cases.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalHematology
Volume12
Issue number1
DOIs
Publication statusPublished - Feb 2007

Fingerprint

P-Glycoproteins
Cytarabine
Drug Resistance
Leukemia
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Pharmaceutical Preparations
Tetrazolium Salts
Daunorubicin
Myeloid Leukemia
In Vitro Techniques

Keywords

  • ABC superfamily
  • Ara-C
  • Daunorubicin
  • MDR proteins
  • MTS assay

ASJC Scopus subject areas

  • Hematology

Cite this

Expression of multidrug resistance (MDR) proteins and in vitro drug resistance in acute leukemias. / Nordin, Fazlina; Maha, A.; A. Jamal, A. Rahman; Abdul Latiff, Zarina; Cheong, S. K.; Hamidah, H.; Ainoon, O.; Syed Zakaria, Syed Zulkifli; Hussin, Noor Hamidah.

In: Hematology, Vol. 12, No. 1, 02.2007, p. 33-37.

Research output: Contribution to journalArticle

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AU - Cheong, S. K.

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