Expresión del fenotipo de células troncales mesenquimales en células epiteliales nasales humanas

Translated title of the contribution: Expression of mesenchymal stem cell phenotype in human nasal respiratory epithelial cells

Aisha A. Muhammed, Mohd Heikal Mohd Yunus, Aminuddin B. Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

Abstract

The respiratory epithelium is the first line of contact with the external hazards. Thus it can be damaged and need to be replaced to avoid healing by fibrosis. Tracheal tissue engineering is an alternative promising treatment modality. Mesenchymal stem cell markers are surface proteins, which are responsible for some of these cells unique properties. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping techniques. Respiratory epithelial cells were cultured using co-culture technique, fibroblasts was removed at confluence leaving respiratory epithelial cells, which were passage further to passage 4. Cells were evaluated for mesenchymal stem cell markers that were CD73, CD90, CD105 and the hematopoietic stem cell marker CD45 at passage 1 (P1) and passage 4 (P4) using Flow cytometry and Immunocytochemistry techniques. Respiratory epithelial cells expressed the mesenchymal stem cell markers at P1 and maintain the expression these markers until P4. Using both techniques, to compare the values of mesenchymal stem cell markers expression at P1 to P4 there was no significant difference. This study indicates that respiratory epithelial cells derived from nasal turbinate retain some of mesenchymal stem cells properties even after serial passages. Both methods of Immunophenotyping are comparable.

Original languageSpanish
Pages (from-to)1476-1482
Number of pages7
JournalInternational Journal of Morphology
Volume33
Issue number4
DOIs
Publication statusPublished - 1 Dec 2015

Fingerprint

Mesenchymal Stromal Cells
Nose
Epithelial Cells
Phenotype
Immunophenotyping
Serial Passage
Respiratory Mucosa
Culture Techniques
Turbinates
Tissue Engineering
Hematopoietic Stem Cells
Coculture Techniques
Flow Cytometry
Membrane Proteins
Fibrosis
Fibroblasts
Immunohistochemistry

Keywords

  • Human nasal respiratory epithelium
  • Immunostaining
  • Mesenchymal stem cells marker
  • Nasal turbinate
  • Tissue engineering

ASJC Scopus subject areas

  • Anatomy

Cite this

Expresión del fenotipo de células troncales mesenquimales en células epiteliales nasales humanas. / Muhammed, Aisha A.; Mohd Yunus, Mohd Heikal; Saim, Aminuddin B.; Idrus, Ruszymah.

In: International Journal of Morphology, Vol. 33, No. 4, 01.12.2015, p. 1476-1482.

Research output: Contribution to journalArticle

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